Detailed information of TA system TA080438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 26764..27323
• Replicon: plasmid pFORC69
• Accession: NZ_CP023062
• Organism: Escherichia coli strain FORC_069

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G4VUF8
• Locus tag: FORC69_RS27415
• Protein ID: WP_000421273.1
• Coordinates: 27048..27323 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: FORC69_RS27410
• Protein ID: WP_001387467.1
• Coordinates: 26764..27048 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC69_RS27355	22472..22684	+	213	WP_001388588.1	ANR family transcriptional regulator	-
FORC69_RS27360	22929..23390	+	462	WP_001233835.1	thermonuclease family protein	-
FORC69_RS27365	23436..23645	+	210	Protein_34	hemolysin expression modulator Hha	-
FORC69_RS27370	23684..24274	+	591	WP_000766804.1	DUF2726 domain-containing protein	-
FORC69_RS27375	24514..24771	+	258	WP_000083826.1	replication regulatory protein RepA	-
FORC69_RS27385	25004..25078	+	75	WP_001388587.1	RepA leader peptide Tap	-
FORC69_RS27390	25071..25604	+	534	WP_001505108.1	incFII RepA family protein	-
FORC69_RS27395	25594..26214	+	621	Protein_39	replication initiation protein	-
FORC69_RS27410	26764..27048	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
FORC69_RS27415	27048..27323	+	276	WP_000421273.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC69_RS27420	27429..27722	+	294	WP_001105065.1	hypothetical protein	-
FORC69_RS27425	27757..27885	-	129	Protein_43	IS481 family transposase	-
FORC69_RS27430	27918..29086	-	1169	Protein_44	hypothetical protein	-
FORC69_RS27440	29579..29886	+	308	Protein_45	type II toxin-antitoxin system ParD family antitoxin	-
FORC69_RS27450	30230..30412	+	183	WP_048657295.1	hypothetical protein	-
FORC69_RS27455	30533..31273	+	741	WP_001388582.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	agg3D / agg3C / agg3B / agg3A / aap/aspU / pic	1..77306	77306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.45 Da
• Isoelectric Point: 9.9324

>T80438 WP_000421273.1 NZ_CP023062:27048-27323 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T80438 NZ_CP023062:27048-27323 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT80438 WP_001387467.1 NZ_CP023062:26764-27048 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT80438 NZ_CP023062:26764-27048 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6H1

Antitoxin

• AlphaFold DB: A0A0E0Y880