Detailed information of TA system TA080431

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4699433..4700234
• Replicon: chromosome
• Accession: NZ_CP023061
• Organism: Escherichia coli strain FORC_069

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: FORC69_RS24775
• Protein ID: WP_001094430.1
• Coordinates: 4699857..4700234 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FORC69_RS24770
• Protein ID: WP_088888735.1
• Coordinates: 4699433..4699810 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC69_RS24735	4694636..4696144	-	1509	WP_001189118.1	group II intron reverse transcriptase/maturase	-
FORC69_RS24745	4696817..4697556	-	740	Protein_4506	IS3 family transposase	-
FORC69_RS28085	4697613..4697738	+	126	Protein_4507	DUF945 domain-containing protein	-
FORC69_RS24755	4698080..4698553	+	474	WP_000855057.1	antirestriction protein	-
FORC69_RS24760	4698569..4699045	+	477	WP_157787305.1	RadC family protein	-
FORC69_RS24765	4699132..4699353	+	222	WP_000692352.1	DUF987 domain-containing protein	-
FORC69_RS24770	4699433..4699810	+	378	WP_088888735.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FORC69_RS24775	4699857..4700234	+	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
FORC69_RS24780	4700231..4700719	+	489	WP_000761715.1	hypothetical protein	-
FORC69_RS24785	4700739..4700936	+	198	WP_000772029.1	DUF957 domain-containing protein	-
FORC69_RS24790	4701021..4701863	+	843	WP_001290187.1	DUF4942 domain-containing protein	-
FORC69_RS24800	4702612..4704150	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T80431 WP_001094430.1 NZ_CP023061:4699857-4700234 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T80431 NZ_CP023061:4699857-4700234 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.60 Da
• Isoelectric Point: 6.0568

>AT80431 WP_088888735.1 NZ_CP023061:4699433-4699810 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT80431 NZ_CP023061:4699433-4699810 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7