Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 810960..811761 | Replicon | chromosome |
| Accession | NZ_CP023061 | ||
| Organism | Escherichia coli strain FORC_069 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | D3GU15 |
| Locus tag | FORC69_RS04210 | Protein ID | WP_001094429.1 |
| Coordinates | 810960..811337 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D3GU16 |
| Locus tag | FORC69_RS04215 | Protein ID | WP_001285617.1 |
| Coordinates | 811384..811761 (-) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FORC69_RS04175 | 806041..806640 | + | 600 | WP_001255040.1 | type II secretion system minor pseudopilin GspJ | - |
| FORC69_RS04180 | 806643..807620 | + | 978 | WP_000633238.1 | type II secretion system minor pseudopilin GspK | - |
| FORC69_RS04185 | 807617..808795 | + | 1179 | WP_000094962.1 | type II secretion system protein GspL | - |
| FORC69_RS04190 | 808797..809333 | + | 537 | WP_000942795.1 | GspM family type II secretion system protein YghD | - |
| FORC69_RS04195 | 809614..810456 | - | 843 | WP_001280493.1 | DUF4942 domain-containing protein | - |
| FORC69_RS04200 | 810541..810738 | - | 198 | WP_085949090.1 | DUF957 domain-containing protein | - |
| FORC69_RS04205 | 810766..810963 | - | 198 | Protein_766 | hypothetical protein | - |
| FORC69_RS04210 | 810960..811337 | - | 378 | WP_001094429.1 | TA system toxin CbtA family protein | Toxin |
| FORC69_RS04215 | 811384..811761 | - | 378 | WP_001285617.1 | type IV toxin-antitoxin system toxin CbtA | Antitoxin |
| FORC69_RS04220 | 811841..812062 | - | 222 | WP_000692350.1 | DUF987 domain-containing protein | - |
| FORC69_RS04225 | 812149..812625 | - | 477 | WP_001388380.1 | RadC family protein | - |
| FORC69_RS04230 | 812641..813114 | - | 474 | WP_000855081.1 | antirestriction protein | - |
| FORC69_RS04240 | 813416..814234 | - | 819 | WP_001234605.1 | DUF945 domain-containing protein | - |
| FORC69_RS04250 | 814334..814567 | - | 234 | WP_001119729.1 | DUF905 family protein | - |
| FORC69_RS04255 | 814646..815101 | - | 456 | WP_000581502.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / vipA/tssB / vipB/tssC / clbK | 715845..871525 | 155680 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13856.73 Da Isoelectric Point: 6.8601
>T80411 WP_001094429.1 NZ_CP023061:c811337-810960 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ
Download Length: 378 bp
>T80411 NZ_CP023061:c811337-810960 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13715.59 Da Isoelectric Point: 6.6240
>AT80411 WP_001285617.1 NZ_CP023061:c811761-811384 [Escherichia coli]
VSDTLPGTTPPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
VSDTLPGTTPPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
Download Length: 378 bp
>AT80411 NZ_CP023061:c811761-811384 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XVU5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | D3GU16 |