Detailed information of TA system TA080400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 449800..450359
• Replicon: chromosome
• Accession: NZ_CP023055
• Organism: Vibrio anguillarum strain VIB43

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F7YRQ7
• Locus tag: CK207_RS17275
• Protein ID: WP_010320663.1
• Coordinates: 449800..450078 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A233HRF0
• Locus tag: CK207_RS17280
• Protein ID: WP_017040620.1
• Coordinates: 450075..450359 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CK207_RS17235	445296..445649	-	354	WP_019283195.1	IS66 family insertion sequence element accessory protein TnpB	-
CK207_RS17240	445646..445966	-	321	WP_019283194.1	IS66 family insertion sequence hypothetical protein	-
CK207_RS17245	446049..446342	-	294	Protein_402	helix-turn-helix domain-containing protein	-
CK207_RS17250	446998..447783	-	786	WP_019283218.1	DUF4225 domain-containing protein	-
CK207_RS17255	447776..448269	-	494	Protein_404	Hcp family type VI secretion system effector	-
CK207_RS17265	449179..449658	+	480	WP_017045717.1	lecithin retinol acyltransferase family protein	-
CK207_RS17275	449800..450078	-	279	WP_010320663.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CK207_RS17280	450075..450359	-	285	WP_017040620.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CK207_RS17285	450557..451062	+	506	Protein_408	lipocalin family protein	-
CK207_RS17295	452518..452760	+	243	WP_095661368.1	DUF1289 domain-containing protein	-
CK207_RS17300	452880..453110	-	231	WP_013868597.1	PAS factor family protein	-
CK207_RS17310	453414..453701	+	288	WP_029388214.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CK207_RS17315	453712..454029	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
CK207_RS17325	454178..454594	+	417	WP_017045395.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	386109..446342	60233
	-	inside	IScluster/Tn	-	-	451369..475643	24274
	-	inside	Integron	-	-	446572..456331	9759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-44)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10878.58 Da
• Isoelectric Point: 4.3430

>T80400 WP_010320663.1 NZ_CP023055:c450078-449800 [Vibrio anguillarum]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDDIIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVEGSIIRIM
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T80400 NZ_CP023055:c450078-449800 [Vibrio anguillarum]
ATGATTTTATGGGAAGAAGAATCGCTAAATGATCGTGAGAAGATCTTTGAGTTTCTTTATGACTTTAACCCTGATGCGGC
TGAAAAAACTGATGACATTATTGAGGCTAAAGTAGAAAACTTACTCGAACAACCATTGATGGGTGTTCAACGGGATGGAA
TTCGAGGACGATTGCTCATTATTCCCGAAATCTCAATGATCGTTTCGTACTGGGTAGAAGGTTCAATTATTCGCATAATG
CGAGTTCTACACCAGAAGCAGAAATTCCCTACCGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10957.29 Da
• Isoelectric Point: 9.2173

>AT80400 WP_017040620.1 NZ_CP023055:c450359-450075 [Vibrio anguillarum]
MDTRIQFRVDDEIKRLAQQMAESQGRTLSDACRELTEQMAEQQRKTLSHDAWLTEQINLAFEKFDSGKSSFVEHNSAKAR
MAERKAKIRNRGQQ

Nucleotide

• Length: 285 bp

>AT80400 NZ_CP023055:c450359-450075 [Vibrio anguillarum]
ATGGACACTAGAATTCAATTTCGTGTTGATGACGAAATTAAACGTTTAGCCCAACAAATGGCTGAGAGCCAAGGCCGCAC
ACTCAGCGATGCTTGCCGTGAACTTACTGAACAAATGGCTGAGCAACAAAGAAAAACATTATCTCATGACGCTTGGTTAA
CTGAACAAATAAATCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTTCATTTGTTGAACATAATTCAGCTAAAGCACGA
ATGGCTGAACGAAAGGCCAAAATTCGTAATCGAGGCCAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A0F2IQF3

Antitoxin

• AlphaFold DB: A0A233HRF0