Detailed information of TA system TA080399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 432026..432792
• Replicon: chromosome
• Accession: NZ_CP023055
• Organism: Vibrio anguillarum strain VIB43

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A290PTK1
• Locus tag: CK207_RS17135
• Protein ID: WP_017045788.1
• Coordinates: 432026..432523 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A290Q6J3
• Locus tag: CK207_RS17140
• Protein ID: WP_019283269.1
• Coordinates: 432520..432792 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CK207_RS17100	427232..427747	+	516	WP_019283453.1	peptide deformylase	-
CK207_RS17115	429538..430251	+	714	WP_019283452.1	Fic family protein	-
CK207_RS20760	430248..430358	+	111	Protein_379	DUF3265 domain-containing protein	-
CK207_RS17120	430408..430785	+	378	WP_019283451.1	hypothetical protein	-
CK207_RS17125	430901..431869	+	969	WP_081245536.1	IS30 family transposase	-
CK207_RS17130	431914..432003	+	90	Protein_382	hypothetical protein	-
CK207_RS17135	432026..432523	-	498	WP_017045788.1	GNAT family N-acetyltransferase	Toxin
CK207_RS17140	432520..432792	-	273	WP_019283269.1	DUF1778 domain-containing protein	Antitoxin
CK207_RS17145	432832..432993	+	162	WP_019283270.1	DUF3265 domain-containing protein	-
CK207_RS17155	433542..433961	+	420	WP_069212067.1	hypothetical protein	-
CK207_RS17160	435102..435552	+	451	Protein_387	effector binding domain-containing protein	-
CK207_RS17165	435696..436217	+	522	WP_019283291.1	GNAT family N-acetyltransferase	-
CK207_RS17170	436549..436869	+	321	WP_019283194.1	IS66 family insertion sequence hypothetical protein	-
CK207_RS17175	436866..437219	+	354	WP_019283195.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	386109..446342	60233
	-	inside	Integron	-	-	427232..442955	15723

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18524.30 Da
• Isoelectric Point: 9.2813

>T80399 WP_017045788.1 NZ_CP023055:c432523-432026 [Vibrio anguillarum]
MMKTVLLDKDKHDRNRFNCGIDALNNYLKVMASQQAKKDNTRTFVLEDENNTSLIIGFYTLTMTPIDLKTLPDKLQKKHQ
SSTSGGLIARLAIDDSYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFKAFQDAENKLFITISDI
RASLG

Nucleotide

• Length: 498 bp

>T80399 NZ_CP023055:c432523-432026 [Vibrio anguillarum]
ATGATGAAAACGGTATTACTCGATAAAGATAAACACGACAGAAACCGATTCAACTGCGGAATTGATGCGCTTAATAACTA
TTTGAAAGTCATGGCAAGTCAGCAAGCTAAAAAAGATAATACCCGAACATTTGTTTTAGAGGATGAAAATAACACCTCAC
TTATCATCGGTTTTTACACTTTAACAATGACACCCATTGACCTAAAAACATTGCCTGATAAGTTACAGAAAAAGCATCAG
TCATCAACTTCTGGCGGACTCATTGCTCGCTTAGCTATCGATGATAGCTATAAAGGTAAAGGATTTGGCGAGTGGTTACT
TATCGATGCTCTACGAAAACTACTAGCAGCTAGTGATAGCGTGGCATTTCCCGTCGTTATTGTTGACGCAAAAGATGGCG
CCAAACATTTCTATGAGCGCTATGGTTTTAAAGCGTTTCAAGATGCAGAAAATAAGCTATTTATCACCATTTCAGATATT
AGGGCAAGTTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9917.47 Da
• Isoelectric Point: 9.8130

>AT80399 WP_019283269.1 NZ_CP023055:c432792-432520 [Vibrio anguillarum]
MATTLPRITARVDVDTQNLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQTLKLSQTDAILLMEALDKPTTLNQKLKT
AAERYETRTQ

Nucleotide

• Length: 273 bp

>AT80399 NZ_CP023055:c432792-432520 [Vibrio anguillarum]
ATGGCTACCACTTTACCTCGTATCACTGCGAGAGTTGATGTCGATACACAAAACTTATTAACCAAAGCGGCTGCTCTCGC
TGGCATGTCTAGTATTAACTCATTTGTACTCAATGCTGCAATCGAAAAAGCAAAGCAAGTGATTGAACGCGAACAAACAC
TAAAATTAAGCCAAACAGACGCCATTTTACTTATGGAAGCTCTCGATAAACCAACAACATTAAATCAAAAGCTTAAAACT
GCCGCCGAACGCTATGAGACCAGAACCCAATGA

Structures

Toxin

• AlphaFold DB: A0A290PTK1

Antitoxin

• AlphaFold DB: A0A290Q6J3