Detailed information of TA system TA080397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 29411..29988
• Replicon: plasmid pCFSAN021143
• Accession: NZ_CP023053
• Organism: Listeria monocytogenes strain FDA709873-31

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: ARX13_RS15935
• Protein ID: WP_003728465.1
• Coordinates: 29411..29752 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: ARX13_RS15940
• Protein ID: WP_003728464.1
• Coordinates: 29746..29988 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARX13_RS15920	25890..26444	-	555	WP_003728468.1	recombinase family protein	-
ARX13_RS15925	26724..27083	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
ARX13_RS15930	27083..29218	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
ARX13_RS15935	29411..29752	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ARX13_RS15940	29746..29988	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ARX13_RS15945	30049..30300	-	252	WP_003728463.1	hypothetical protein	-
ARX13_RS15950	30431..30757	+	327	WP_003728462.1	hypothetical protein	-
ARX13_RS15955	30757..31419	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
ARX13_RS15960	31438..31815	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
ARX13_RS16250	31974..32462	+	489	WP_003728520.1	hypothetical protein	-
ARX13_RS15970	32584..32832	+	249	WP_003728519.1	hypothetical protein	-
ARX13_RS15975	32833..33258	+	426	WP_003728518.1	hypothetical protein	-
ARX13_RS15980	33262..33984	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
ARX13_RS15985	34000..34716	+	717	WP_003731678.1	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61919	61919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T80397 WP_003728465.1 NZ_CP023053:c29752-29411 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T80397 NZ_CP023053:c29752-29411 [Listeria monocytogenes]
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Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT80397 WP_003728464.1 NZ_CP023053:c29988-29746 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT80397 NZ_CP023053:c29988-29746 [Listeria monocytogenes]
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TTT

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5