Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 880469..881008 Replicon chromosome
Accession NZ_CP023047
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-186

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag CKG20_RS04460 Protein ID WP_006247804.1
Coordinates 880469..880738 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag CKG20_RS04465 Protein ID WP_006247803.1
Coordinates 880742..881008 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG20_RS04415 875603..875902 + 300 WP_006247810.1 XRE family transcriptional regulator -
CKG20_RS14205 875911..876069 - 159 WP_006247809.1 hypothetical protein -
CKG20_RS04420 876079..876765 - 687 WP_006247808.1 hypothetical protein -
CKG20_RS04425 876876..877487 - 612 WP_006253310.1 hypothetical protein -
CKG20_RS04430 877536..878162 - 627 WP_006247807.1 tail assembly protein -
CKG20_RS04440 878384..879103 - 720 WP_006247806.1 preprotein translocase subunit YajC -
CKG20_RS04445 879093..879383 - 291 Protein_831 C40 family peptidase -
CKG20_RS04450 879361..880071 - 711 WP_006253312.1 tape measure protein -
CKG20_RS04455 880131..880394 - 264 WP_006247805.1 hypothetical protein -
CKG20_RS04460 880469..880738 - 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
CKG20_RS04465 880742..881008 - 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CKG20_RS04470 881076..881306 - 231 WP_006247802.1 DUF4035 domain-containing protein -
CKG20_RS04475 881351..881752 - 402 WP_006247801.1 hypothetical protein -
CKG20_RS04480 881835..882476 - 642 WP_006247800.1 hypothetical protein -
CKG20_RS04485 882508..882903 - 396 WP_006247799.1 phage tail protein -
CKG20_RS04490 882928..883155 - 228 Protein_840 phage terminase large subunit family protein -
CKG20_RS04495 883305..883679 + 375 WP_006247798.1 hypothetical protein -
CKG20_RS04500 883687..883854 + 168 WP_006253314.1 DNA cytosine methyltransferase -
CKG20_RS04505 883935..884258 + 324 WP_006253316.1 helix-turn-helix domain-containing protein -
CKG20_RS04510 884162..884872 + 711 Protein_844 tyrosine-type recombinase/integrase -
CKG20_RS04515 884973..885608 + 636 Protein_845 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 873128..894915 21787
- flank IS/Tn - - 884973..885665 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T80392 WP_006247804.1 NZ_CP023047:c880738-880469 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T80392 NZ_CP023047:c880738-880469 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT80392 WP_006247803.1 NZ_CP023047:c881008-880742 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT80392 NZ_CP023047:c881008-880742 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References