Detailed information of TA system TA080391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 858001..858597
• Replicon: chromosome
• Accession: NZ_CP023047
• Organism: Mannheimia haemolytica strain USDA-ARS-USMARC-186

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CKG20_RS04330
• Protein ID: WP_006249776.1
• Coordinates: 858373..858597 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A547EKP0
• Locus tag: CKG20_RS04325
• Protein ID: WP_006249777.1
• Coordinates: 858001..858330 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKG20_RS04300	853450..854139	+	690	WP_006253589.1	GTP cyclohydrolase I FolE	-
CKG20_RS04305	854204..854989	+	786	WP_006249781.1	tRNA pseudouridine(38-40) synthase TruA	-
CKG20_RS04310	855042..857234	-	2193	WP_006249780.1	copper-translocating P-type ATPase	-
CKG20_RS04315	857287..857487	-	201	WP_006249779.1	heavy-metal-associated domain-containing protein	-
CKG20_RS04320	857576..857962	+	387	WP_006249778.1	Cu(I)-responsive transcriptional regulator	-
CKG20_RS04325	858001..858330	-	330	WP_006249777.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CKG20_RS04330	858373..858597	-	225	WP_006249776.1	type II toxin-antitoxin system HicA family toxin	Toxin
CKG20_RS04335	858805..860712	+	1908	WP_006249774.1	DNA topoisomerase IV subunit B	-
CKG20_RS04340	860786..861391	-	606	WP_006249773.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
CKG20_RS04345	861461..861817	+	357	WP_006249772.1	dihydroneopterin aldolase	-
CKG20_RS04350	861814..862716	+	903	WP_006249771.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (28-73)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 10.5597

>T80391 WP_006249776.1 NZ_CP023047:c858597-858373 [Mannheimia haemolytica]
MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGSRIAVVLFQQVRVFHRPHPNPDTDKGR

Nucleotide

• Length: 225 bp

>T80391 NZ_CP023047:c858597-858373 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12312.06 Da
• Isoelectric Point: 4.7022

>AT80391 WP_006249777.1 NZ_CP023047:c858330-858001 [Mannheimia haemolytica]
MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQLKKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSP
ELHKAAVIAATAENLSLNEWINQTLEKSV

Nucleotide

• Length: 330 bp

>AT80391 NZ_CP023047:c858330-858001 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A547EKP0