Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 304639..305228 Replicon chromosome
Accession NZ_CP023047
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-186

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag CKG20_RS01565 Protein ID WP_006248516.1
Coordinates 304639..304971 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag CKG20_RS01570 Protein ID WP_006248517.1
Coordinates 304968..305228 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG20_RS01525 299834..300454 - 621 WP_006248508.1 dTMP kinase -
CKG20_RS01530 300465..301499 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
CKG20_RS01550 302079..302606 + 528 WP_006248512.1 inorganic diphosphatase -
CKG20_RS01555 302662..303417 + 756 WP_006248513.1 M48 family metallopeptidase -
CKG20_RS01560 303481..304512 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
CKG20_RS01565 304639..304971 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CKG20_RS01570 304968..305228 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CKG20_RS01575 305298..305966 - 669 WP_006248518.1 phosphoglycolate phosphatase -
CKG20_RS01580 306053..306850 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
CKG20_RS01585 306858..307646 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
CKG20_RS01590 307648..308292 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
CKG20_RS01595 308904..310049 + 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T80389 WP_006248516.1 NZ_CP023047:c304971-304639 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T80389 NZ_CP023047:c304971-304639 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT80389 WP_006248517.1 NZ_CP023047:c305228-304968 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT80389 NZ_CP023047:c305228-304968 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References