Detailed information of TA system TA080388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 76478..77193
• Replicon: chromosome
• Accession: NZ_CP023047
• Organism: Mannheimia haemolytica strain USDA-ARS-USMARC-186

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CKG20_RS00375
• Protein ID: WP_006250491.1
• Coordinates: 76478..76906 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: CKG20_RS00380
• Protein ID: WP_006248272.1
• Coordinates: 76906..77193 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKG20_RS00360	71732..73384	+	1653	WP_006248275.1	phospho-sugar mutase	-
CKG20_RS00365	73437..74371	+	935	Protein_73	nucleoside hydrolase	-
CKG20_RS00370	74393..76474	-	2082	WP_006248274.1	ATP-dependent DNA helicase RecG	-
CKG20_RS00375	76478..76906	-	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
CKG20_RS00380	76906..77193	-	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CKG20_RS00385	77357..79240	+	1884	WP_006248270.1	molecular chaperone HtpG	-
CKG20_RS00390	79618..81333	+	1716	WP_006248268.1	proline--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T80388 WP_006250491.1 NZ_CP023047:c76906-76478 [Mannheimia haemolytica]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T80388 NZ_CP023047:c76906-76478 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT80388 WP_006248272.1 NZ_CP023047:c77193-76906 [Mannheimia haemolytica]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT80388 NZ_CP023047:c77193-76906 [Mannheimia haemolytica]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87