Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1917817..1918339 Replicon chromosome
Accession NZ_CP023046
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-187

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag CKG21_RS09950 Protein ID WP_006249269.1
Coordinates 1918043..1918339 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag CKG21_RS09945 Protein ID WP_006249270.1
Coordinates 1917817..1918059 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG21_RS09920 1913655..1914744 - 1090 Protein_1930 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
CKG21_RS09925 1914918..1915559 - 642 WP_006249274.1 orotate phosphoribosyltransferase -
CKG21_RS09930 1915682..1916524 - 843 WP_006249273.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
CKG21_RS09935 1916584..1917159 + 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
CKG21_RS09940 1917137..1917805 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
CKG21_RS09945 1917817..1918059 - 243 WP_006249270.1 CopG family transcriptional regulator Antitoxin
CKG21_RS09950 1918043..1918339 - 297 WP_006249269.1 BrnT family toxin Toxin
CKG21_RS09955 1918466..1921378 - 2913 WP_006249267.1 RNA polymerase-associated protein RapA -
CKG21_RS09960 1921539..1921838 - 300 WP_006249265.1 hypothetical protein -
CKG21_RS09965 1921959..1923284 - 1326 WP_006249264.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - htrB 1897089..2052393 155304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T80386 WP_006249269.1 NZ_CP023046:c1918339-1918043 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T80386 NZ_CP023046:c1918339-1918043 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT80386 WP_006249270.1 NZ_CP023046:c1918059-1917817 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT80386 NZ_CP023046:c1918059-1917817 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References