Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1698310..1698849 Replicon chromosome
Accession NZ_CP023046
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-187

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag CKG21_RS08775 Protein ID WP_006247804.1
Coordinates 1698580..1698849 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag CKG21_RS08770 Protein ID WP_006247803.1
Coordinates 1698310..1698576 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG21_RS08720 1693710..1694345 - 636 Protein_1678 transposase -
CKG21_RS08725 1694446..1695156 - 711 Protein_1679 tyrosine-type recombinase/integrase -
CKG21_RS08730 1695060..1695383 - 324 WP_095586246.1 helix-turn-helix domain-containing protein -
CKG21_RS08735 1695464..1695631 - 168 WP_006253314.1 DNA cytosine methyltransferase -
CKG21_RS08740 1695639..1696013 - 375 WP_006247798.1 hypothetical protein -
CKG21_RS08745 1696163..1696390 + 228 Protein_1683 phage terminase large subunit family protein -
CKG21_RS08750 1696415..1696810 + 396 WP_006247799.1 phage tail protein -
CKG21_RS08755 1696842..1697483 + 642 WP_006247800.1 hypothetical protein -
CKG21_RS08760 1697566..1697967 + 402 WP_006247801.1 hypothetical protein -
CKG21_RS08765 1698012..1698242 + 231 WP_006247802.1 DUF4035 domain-containing protein -
CKG21_RS08770 1698310..1698576 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CKG21_RS08775 1698580..1698849 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
CKG21_RS08780 1698924..1699187 + 264 WP_006247805.1 hypothetical protein -
CKG21_RS08785 1699247..1699957 + 711 WP_006253312.1 tape measure protein -
CKG21_RS08790 1699935..1700225 + 291 Protein_1692 C40 family peptidase -
CKG21_RS08795 1700215..1700934 + 720 WP_006247806.1 preprotein translocase subunit YajC -
CKG21_RS08805 1701156..1701782 + 627 WP_006247807.1 tail assembly protein -
CKG21_RS08810 1701831..1702442 + 612 WP_006253310.1 hypothetical protein -
CKG21_RS08815 1702553..1703239 + 687 WP_006247808.1 hypothetical protein -
CKG21_RS14210 1703249..1703407 + 159 WP_006247809.1 hypothetical protein -
CKG21_RS08820 1703416..1703715 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1684403..1747509 63106
- flank IS/Tn - - 1693653..1694345 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T80385 WP_006247804.1 NZ_CP023046:1698580-1698849 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T80385 NZ_CP023046:1698580-1698849 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT80385 WP_006247803.1 NZ_CP023046:1698310-1698576 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT80385 NZ_CP023046:1698310-1698576 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References