Detailed information of TA system TA080384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1479601..1480241
• Replicon: chromosome
• Accession: NZ_CP023046
• Organism: Mannheimia haemolytica strain USDA-ARS-USMARC-187

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: CKG21_RS07605
• Protein ID: WP_006250075.1
• Coordinates: 1479601..1479783 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: CKG21_RS07610
• Protein ID: WP_006250074.1
• Coordinates: 1479822..1480241 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKG21_RS07560	1475148..1475441	-	294	WP_006247812.1	hypothetical protein	-
CKG21_RS07565	1475585..1475974	+	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CKG21_RS07570	1475946..1476245	+	300	WP_006247810.1	XRE family transcriptional regulator	-
CKG21_RS14190	1476254..1476412	-	159	WP_006247809.1	hypothetical protein	-
CKG21_RS14475	1476422..1477489	-	1068	WP_006253284.1	hypothetical protein	-
CKG21_RS07585	1477510..1477710	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
CKG21_RS07590	1477841..1478524	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
CKG21_RS07595	1478599..1478979	+	381	WP_006248823.1	hypothetical protein	-
CKG21_RS07600	1478984..1479469	+	486	WP_015484250.1	hypothetical protein	-
CKG21_RS07605	1479601..1479783	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
CKG21_RS07610	1479822..1480241	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CKG21_RS07615	1480577..1481221	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
CKG21_RS07620	1481359..1482405	+	1047	WP_020849882.1	IS481 family transposase	-
CKG21_RS07625	1482454..1484685	-	2232	WP_006253498.1	collagen-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1465391..1562091	96700
	-	flank	IS/Tn	-	-	1481359..1482405	1046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T80384 WP_006250075.1 NZ_CP023046:1479601-1479783 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T80384 NZ_CP023046:1479601-1479783 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT80384 WP_006250074.1 NZ_CP023046:1479822-1480241 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT80384 NZ_CP023046:1479822-1480241 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9