Detailed information of TA system TA080383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1145119..1145767
• Replicon: chromosome
• Accession: NZ_CP023046
• Organism: Mannheimia haemolytica strain USDA-ARS-USMARC-187

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: CKG21_RS05970
• Protein ID: WP_006251221.1
• Coordinates: 1145591..1145767 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: CKG21_RS05965
• Protein ID: WP_006251222.1
• Coordinates: 1145119..1145535 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKG21_RS05915	1140204..1140638	+	435	WP_006251230.1	hypothetical protein	-
CKG21_RS05920	1140653..1140889	+	237	Protein_1127	encapsulating for peroxidase	-
CKG21_RS05925	1140948..1141988	-	1041	WP_020910232.1	IS481 family transposase	-
CKG21_RS14155	1141987..1142088	+	102	Protein_1129	DNA primase	-
CKG21_RS05930	1142089..1142850	+	762	Protein_1130	encapsulin	-
CKG21_RS05935	1142862..1143122	+	261	WP_020824317.1	hypothetical protein	-
CKG21_RS05940	1143125..1143502	+	378	WP_006251227.1	hypothetical protein	-
CKG21_RS05945	1143502..1143846	+	345	WP_006251226.1	hypothetical protein	-
CKG21_RS05950	1143848..1144216	+	369	WP_006251225.1	hypothetical protein	-
CKG21_RS05955	1144213..1144593	+	381	WP_006251224.1	hypothetical protein	-
CKG21_RS05960	1144597..1145079	+	483	WP_095586237.1	phage tail protein	-
CKG21_RS05965	1145119..1145535	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CKG21_RS05970	1145591..1145767	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
CKG21_RS05975	1145863..1146534	+	672	WP_006251220.1	hypothetical protein	-
CKG21_RS05980	1146677..1147717	-	1041	WP_015587043.1	IS481 family transposase	-
CKG21_RS05985	1147796..1147990	-	195	WP_095586238.1	hypothetical protein	-
CKG21_RS05990	1148159..1148617	+	459	WP_006251218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1140948..1147717	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T80383 WP_006251221.1 NZ_CP023046:c1145767-1145591 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T80383 NZ_CP023046:c1145767-1145591 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT80383 WP_006251222.1 NZ_CP023046:c1145535-1145119 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT80383 NZ_CP023046:c1145535-1145119 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6