Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 796078..796674 Replicon chromosome
Accession NZ_CP023046
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-187

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag CKG21_RS04020 Protein ID WP_006249776.1
Coordinates 796450..796674 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A547EKP0
Locus tag CKG21_RS04015 Protein ID WP_006249777.1
Coordinates 796078..796407 (-) Length 110 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG21_RS03990 791527..792216 + 690 WP_006253589.1 GTP cyclohydrolase I FolE -
CKG21_RS03995 792281..793066 + 786 WP_006249781.1 tRNA pseudouridine(38-40) synthase TruA -
CKG21_RS04000 793119..795311 - 2193 WP_006249780.1 copper-translocating P-type ATPase -
CKG21_RS04005 795364..795564 - 201 WP_006249779.1 heavy-metal-associated domain-containing protein -
CKG21_RS04010 795653..796039 + 387 WP_006249778.1 Cu(I)-responsive transcriptional regulator -
CKG21_RS04015 796078..796407 - 330 WP_006249777.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CKG21_RS04020 796450..796674 - 225 WP_006249776.1 type II toxin-antitoxin system HicA family toxin Toxin
CKG21_RS04025 796882..798789 + 1908 WP_006249774.1 DNA topoisomerase IV subunit B -
CKG21_RS04030 798863..799468 - 606 WP_006249773.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
CKG21_RS04035 799538..799894 + 357 WP_006249772.1 dihydroneopterin aldolase -
CKG21_RS04040 799891..800793 + 903 WP_006249771.1 DMT family transporter -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(28-73)

Antitoxin

(55-105)


Sequences


Toxin        


Download         Length: 75 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 10.5597

>T80382 WP_006249776.1 NZ_CP023046:c796674-796450 [Mannheimia haemolytica]
MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGSRIAVVLFQQVRVFHRPHPNPDTDKGR

Download         Length: 225 bp

>T80382 NZ_CP023046:c796674-796450 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12312.06 Da        Isoelectric Point: 4.7022

>AT80382 WP_006249777.1 NZ_CP023046:c796407-796078 [Mannheimia haemolytica]
MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQLKKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSP
ELHKAAVIAATAENLSLNEWINQTLEKSV

Download         Length: 330 bp

>AT80382 NZ_CP023046:c796407-796078 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A547EKP0

References