Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2281718..2282368 Replicon chromosome
Accession NZ_CP023044
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-191

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag CKG22_RS11870 Protein ID WP_006249411.1
Coordinates 2281718..2282107 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A547EDM1
Locus tag CKG22_RS11875 Protein ID WP_006249412.1
Coordinates 2282108..2282368 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG22_RS11825 2277196..2278005 - 810 WP_006250123.1 metal ABC transporter permease -
CKG22_RS11830 2277998..2278858 - 861 WP_006250124.1 metal ABC transporter permease -
CKG22_RS11835 2278932..2279717 - 786 WP_006251768.1 hypothetical protein -
CKG22_RS11865 2280460..2281698 - 1239 WP_006253630.1 peptidase T -
CKG22_RS11870 2281718..2282107 - 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
CKG22_RS11875 2282108..2282368 - 261 WP_006249412.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CKG22_RS11880 2282484..2282696 - 213 WP_006251765.1 BrnA antitoxin family protein -
CKG22_RS11885 2282697..2282984 - 288 WP_006251764.1 BrnT family toxin -
CKG22_RS11890 2283147..2286680 - 3534 WP_006253629.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T80378 WP_006249411.1 NZ_CP023044:c2282107-2281718 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T80378 NZ_CP023044:c2282107-2281718 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGTGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.54 Da        Isoelectric Point: 4.8951

>AT80378 WP_006249412.1 NZ_CP023044:c2282368-2282108 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT80378 NZ_CP023044:c2282368-2282108 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAAAAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A547EDM1

References