80376

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1494854..1495502 Replicon chromosome
Accession NZ_CP023044
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-191

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag CKG22_RS07685 Protein ID WP_006251221.1
Coordinates 1494854..1495030 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag CKG22_RS07690 Protein ID WP_006251222.1
Coordinates 1495086..1495502 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG22_RS07660 1490172..1492667 - 2496 WP_006251216.1 phage tail length tape measure family protein -
CKG22_RS07670 1493212..1493670 - 459 WP_006251218.1 hypothetical protein -
CKG22_RS07675 1493839..1494072 + 234 WP_020824316.1 hypothetical protein -
CKG22_RS07680 1494087..1494758 - 672 WP_006251220.1 hypothetical protein -
CKG22_RS07685 1494854..1495030 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
CKG22_RS07690 1495086..1495502 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CKG22_RS07695 1495542..1496024 - 483 WP_006251223.1 phage tail protein -
CKG22_RS07700 1496028..1496408 - 381 WP_006251224.1 hypothetical protein -
CKG22_RS07705 1496405..1496773 - 369 WP_006251225.1 hypothetical protein -
CKG22_RS07710 1496775..1497119 - 345 WP_006251226.1 hypothetical protein -
CKG22_RS07715 1497119..1497496 - 378 WP_006251227.1 hypothetical protein -
CKG22_RS07720 1497499..1497777 - 279 WP_021279981.1 hypothetical protein -
CKG22_RS07725 1497789..1498778 - 990 WP_006251229.1 encapsulin -
CKG22_RS07730 1498793..1499227 - 435 WP_006251230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1471068..1554853 83785


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T80376 WP_006251221.1 NZ_CP023044:1494854-1495030 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T80376 NZ_CP023044:1494854-1495030 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT80376 WP_006251222.1 NZ_CP023044:1495086-1495502 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT80376 NZ_CP023044:1495086-1495502 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References