Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 883708..884247 Replicon chromosome
Accession NZ_CP023044
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-191

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag CKG22_RS04480 Protein ID WP_006247804.1
Coordinates 883708..883977 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag CKG22_RS04485 Protein ID WP_006247803.1
Coordinates 883981..884247 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG22_RS04435 878842..879141 + 300 WP_006247810.1 XRE family transcriptional regulator -
CKG22_RS14215 879150..879308 - 159 WP_006247809.1 hypothetical protein -
CKG22_RS04440 879318..880004 - 687 WP_006247808.1 hypothetical protein -
CKG22_RS04445 880115..880726 - 612 WP_006253310.1 hypothetical protein -
CKG22_RS04450 880775..881401 - 627 WP_006247807.1 tail assembly protein -
CKG22_RS04460 881623..882342 - 720 WP_006247806.1 preprotein translocase subunit YajC -
CKG22_RS04465 882332..882622 - 291 Protein_834 C40 family peptidase -
CKG22_RS04470 882600..883310 - 711 WP_006253312.1 tape measure protein -
CKG22_RS04475 883370..883633 - 264 WP_006247805.1 hypothetical protein -
CKG22_RS04480 883708..883977 - 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
CKG22_RS04485 883981..884247 - 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CKG22_RS04490 884315..884545 - 231 WP_006247802.1 DUF4035 domain-containing protein -
CKG22_RS04495 884590..884991 - 402 WP_006247801.1 hypothetical protein -
CKG22_RS04500 885074..885715 - 642 WP_006247800.1 hypothetical protein -
CKG22_RS04505 885747..886142 - 396 WP_006247799.1 phage tail protein -
CKG22_RS04510 886167..886394 - 228 Protein_843 phage terminase large subunit family protein -
CKG22_RS04515 886544..886918 + 375 WP_006247798.1 hypothetical protein -
CKG22_RS04520 886926..887093 + 168 WP_006253314.1 DNA cytosine methyltransferase -
CKG22_RS04525 887174..887497 + 324 WP_006253316.1 helix-turn-helix domain-containing protein -
CKG22_RS04530 887401..888111 + 711 Protein_847 tyrosine-type recombinase/integrase -
CKG22_RS04535 888212..888847 + 636 Protein_848 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 872654..898154 25500
- flank IS/Tn - - 888212..888904 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T80375 WP_006247804.1 NZ_CP023044:c883977-883708 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T80375 NZ_CP023044:c883977-883708 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT80375 WP_006247803.1 NZ_CP023044:c884247-883981 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT80375 NZ_CP023044:c884247-883981 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References