Detailed information of TA system TA080374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 861240..861836
• Replicon: chromosome
• Accession: NZ_CP023044
• Organism: Mannheimia haemolytica strain USDA-ARS-USMARC-191

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CKG22_RS04350
• Protein ID: WP_006249776.1
• Coordinates: 861612..861836 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A547EKP0
• Locus tag: CKG22_RS04345
• Protein ID: WP_006249777.1
• Coordinates: 861240..861569 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKG22_RS04320	856689..857378	+	690	WP_006253589.1	GTP cyclohydrolase I FolE	-
CKG22_RS04325	857443..858228	+	786	WP_006249781.1	tRNA pseudouridine(38-40) synthase TruA	-
CKG22_RS04330	858281..860473	-	2193	WP_006249780.1	copper-translocating P-type ATPase	-
CKG22_RS04335	860526..860726	-	201	WP_006249779.1	heavy-metal-associated domain-containing protein	-
CKG22_RS04340	860815..861201	+	387	WP_006249778.1	Cu(I)-responsive transcriptional regulator	-
CKG22_RS04345	861240..861569	-	330	WP_006249777.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CKG22_RS04350	861612..861836	-	225	WP_006249776.1	type II toxin-antitoxin system HicA family toxin	Toxin
CKG22_RS04355	862044..863951	+	1908	WP_006249774.1	DNA topoisomerase IV subunit B	-
CKG22_RS04360	864025..864630	-	606	WP_006249773.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
CKG22_RS04365	864700..865056	+	357	WP_006249772.1	dihydroneopterin aldolase	-
CKG22_RS04370	865053..865955	+	903	WP_006249771.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (28-73)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 10.5597

>T80374 WP_006249776.1 NZ_CP023044:c861836-861612 [Mannheimia haemolytica]
MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGSRIAVVLFQQVRVFHRPHPNPDTDKGR

Nucleotide

• Length: 225 bp

>T80374 NZ_CP023044:c861836-861612 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12312.06 Da
• Isoelectric Point: 4.7022

>AT80374 WP_006249777.1 NZ_CP023044:c861569-861240 [Mannheimia haemolytica]
MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQLKKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSP
ELHKAAVIAATAENLSLNEWINQTLEKSV

Nucleotide

• Length: 330 bp

>AT80374 NZ_CP023044:c861569-861240 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A547EKP0