Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2213183..2213833 Replicon chromosome
Accession NZ_CP023043
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-193

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag CKG23_RS11540 Protein ID WP_006249411.1
Coordinates 2213183..2213572 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A547EDM1
Locus tag CKG23_RS11545 Protein ID WP_006249412.1
Coordinates 2213573..2213833 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG23_RS11495 2208661..2209470 - 810 WP_006250123.1 metal ABC transporter permease -
CKG23_RS11500 2209463..2210323 - 861 WP_006250124.1 metal ABC transporter permease -
CKG23_RS11505 2210397..2211182 - 786 WP_006251768.1 hypothetical protein -
CKG23_RS11535 2211925..2213163 - 1239 WP_006253630.1 peptidase T -
CKG23_RS11540 2213183..2213572 - 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
CKG23_RS11545 2213573..2213833 - 261 WP_006249412.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CKG23_RS11550 2213949..2214161 - 213 WP_006251765.1 BrnA antitoxin family protein -
CKG23_RS11555 2214162..2214449 - 288 WP_006251764.1 BrnT family toxin -
CKG23_RS11560 2214612..2218145 - 3534 WP_006253629.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T80370 WP_006249411.1 NZ_CP023043:c2213572-2213183 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T80370 NZ_CP023043:c2213572-2213183 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGTGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.54 Da        Isoelectric Point: 4.8951

>AT80370 WP_006249412.1 NZ_CP023043:c2213833-2213573 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT80370 NZ_CP023043:c2213833-2213573 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAAAAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A547EDM1

References