Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1921769..1922291 Replicon chromosome
Accession NZ_CP023043
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-193

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag CKG23_RS10025 Protein ID WP_006249269.1
Coordinates 1921995..1922291 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag CKG23_RS10020 Protein ID WP_006249270.1
Coordinates 1921769..1922011 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG23_RS09995 1917607..1918696 - 1090 Protein_1940 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
CKG23_RS10000 1918870..1919511 - 642 WP_006249274.1 orotate phosphoribosyltransferase -
CKG23_RS10005 1919634..1920476 - 843 WP_006249273.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
CKG23_RS10010 1920536..1921111 + 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
CKG23_RS10015 1921089..1921757 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
CKG23_RS10020 1921769..1922011 - 243 WP_006249270.1 CopG family transcriptional regulator Antitoxin
CKG23_RS10025 1921995..1922291 - 297 WP_006249269.1 BrnT family toxin Toxin
CKG23_RS10030 1922418..1925330 - 2913 WP_006249267.1 RNA polymerase-associated protein RapA -
CKG23_RS10035 1925491..1925790 - 300 WP_006249265.1 hypothetical protein -
CKG23_RS10040 1925911..1927236 - 1326 WP_006249264.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T80369 WP_006249269.1 NZ_CP023043:c1922291-1921995 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T80369 NZ_CP023043:c1922291-1921995 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT80369 WP_006249270.1 NZ_CP023043:c1922011-1921769 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT80369 NZ_CP023043:c1922011-1921769 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References