Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1426364..1427012 Replicon chromosome
Accession NZ_CP023043
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-193

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag CKG23_RS07355 Protein ID WP_006251221.1
Coordinates 1426364..1426540 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag CKG23_RS07360 Protein ID WP_006251222.1
Coordinates 1426596..1427012 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG23_RS07330 1421682..1424177 - 2496 WP_006251216.1 phage tail length tape measure family protein -
CKG23_RS07340 1424722..1425180 - 459 WP_006251218.1 hypothetical protein -
CKG23_RS07345 1425349..1425582 + 234 WP_020824316.1 hypothetical protein -
CKG23_RS07350 1425597..1426268 - 672 WP_006251220.1 hypothetical protein -
CKG23_RS07355 1426364..1426540 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
CKG23_RS07360 1426596..1427012 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CKG23_RS07365 1427052..1427534 - 483 WP_006251223.1 phage tail protein -
CKG23_RS07370 1427538..1427918 - 381 WP_006251224.1 hypothetical protein -
CKG23_RS07375 1427915..1428283 - 369 WP_006251225.1 hypothetical protein -
CKG23_RS07380 1428285..1428629 - 345 WP_006251226.1 hypothetical protein -
CKG23_RS07385 1428629..1429006 - 378 WP_006251227.1 hypothetical protein -
CKG23_RS07390 1429009..1429287 - 279 WP_021279981.1 hypothetical protein -
CKG23_RS07395 1429299..1430288 - 990 WP_006251229.1 encapsulin -
CKG23_RS07400 1430303..1430737 - 435 WP_006251230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1406648..1504806 98158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T80368 WP_006251221.1 NZ_CP023043:1426364-1426540 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T80368 NZ_CP023043:1426364-1426540 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT80368 WP_006251222.1 NZ_CP023043:1426596-1427012 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT80368 NZ_CP023043:1426596-1427012 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References