Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 815206..815745 Replicon chromosome
Accession NZ_CP023043
Organism Mannheimia haemolytica strain USDA-ARS-USMARC-193

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag CKG23_RS04135 Protein ID WP_006247804.1
Coordinates 815206..815475 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag CKG23_RS04140 Protein ID WP_006247803.1
Coordinates 815479..815745 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKG23_RS04090 810340..810639 + 300 WP_006247810.1 XRE family transcriptional regulator -
CKG23_RS14220 810648..810806 - 159 WP_006247809.1 hypothetical protein -
CKG23_RS04095 810816..811502 - 687 WP_006247808.1 hypothetical protein -
CKG23_RS04100 811613..812224 - 612 WP_006253310.1 hypothetical protein -
CKG23_RS04105 812273..812899 - 627 WP_006247807.1 tail assembly protein -
CKG23_RS04115 813121..813840 - 720 WP_006247806.1 preprotein translocase subunit YajC -
CKG23_RS04120 813830..814120 - 291 Protein_766 C40 family peptidase -
CKG23_RS04125 814098..814808 - 711 WP_006253312.1 tape measure protein -
CKG23_RS04130 814868..815131 - 264 WP_006247805.1 hypothetical protein -
CKG23_RS04135 815206..815475 - 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
CKG23_RS04140 815479..815745 - 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CKG23_RS04145 815813..816043 - 231 WP_006247802.1 DUF4035 domain-containing protein -
CKG23_RS04150 816088..816489 - 402 WP_006247801.1 hypothetical protein -
CKG23_RS04155 816572..817213 - 642 WP_006247800.1 hypothetical protein -
CKG23_RS04160 817245..817640 - 396 WP_006247799.1 phage tail protein -
CKG23_RS04165 817665..817892 - 228 Protein_775 phage terminase large subunit family protein -
CKG23_RS04170 818042..818416 + 375 WP_006247798.1 hypothetical protein -
CKG23_RS04175 818424..818591 + 168 WP_006253314.1 DNA cytosine methyltransferase -
CKG23_RS04180 818672..818995 + 324 WP_006253316.1 helix-turn-helix domain-containing protein -
CKG23_RS04185 818899..819609 + 711 Protein_779 tyrosine-type recombinase/integrase -
CKG23_RS04190 819710..820345 + 636 Protein_780 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 807865..825737 17872
- flank IS/Tn - - 819710..820402 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T80367 WP_006247804.1 NZ_CP023043:c815475-815206 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T80367 NZ_CP023043:c815475-815206 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT80367 WP_006247803.1 NZ_CP023043:c815745-815479 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT80367 NZ_CP023043:c815745-815479 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References