Detailed information of TA system TA080363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 11257..11972
• Replicon: chromosome
• Accession: NZ_CP023043
• Organism: Mannheimia haemolytica strain USDA-ARS-USMARC-193

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CKG23_RS00045
• Protein ID: WP_006250491.1
• Coordinates: 11257..11685 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: CKG23_RS00050
• Protein ID: WP_006248272.1
• Coordinates: 11685..11972 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKG23_RS00030	6511..8163	+	1653	WP_006248275.1	phospho-sugar mutase	-
CKG23_RS00035	8216..9150	+	935	Protein_6	nucleoside hydrolase	-
CKG23_RS00040	9172..11253	-	2082	WP_006248274.1	ATP-dependent DNA helicase RecG	-
CKG23_RS00045	11257..11685	-	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
CKG23_RS00050	11685..11972	-	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CKG23_RS00055	12136..14019	+	1884	WP_006248270.1	molecular chaperone HtpG	-
CKG23_RS14505	14072..14233	+	162	WP_015483997.1	hypothetical protein	-
CKG23_RS00060	14397..16112	+	1716	WP_006248268.1	proline--tRNA ligase	-
CKG23_RS00065	16215..16400	+	186	WP_095597181.1	PT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T80363 WP_006250491.1 NZ_CP023043:c11685-11257 [Mannheimia haemolytica]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T80363 NZ_CP023043:c11685-11257 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT80363 WP_006248272.1 NZ_CP023043:c11972-11685 [Mannheimia haemolytica]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT80363 NZ_CP023043:c11972-11685 [Mannheimia haemolytica]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87