Detailed information of TA system TA080362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 20919..21484
• Replicon: plasmid unnamed5
• Accession: NZ_CP023041
• Organism: Komagataeibacter saccharivorans strain CV1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CD178_RS17380
• Protein ID: WP_118963923.1
• Coordinates: 20919..21200 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CD178_RS17385
• Protein ID: WP_118963924.1
• Coordinates: 21197..21484 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD178_RS17345	18222..18515	+	294	WP_118963916.1	conjugal transfer protein TraD	-
CD178_RS17350	18580..18843	+	264	WP_118963917.1	conjugal transfer protein TraD	-
CD178_RS17355	18833..19207	+	375	WP_118963918.1	hypothetical protein	-
CD178_RS17360	19222..19554	+	333	WP_118963919.1	hypothetical protein	-
CD178_RS17365	19558..19815	+	258	WP_118963920.1	conjugal transfer protein TraD	-
CD178_RS17370	19781..20284	+	504	WP_118963921.1	MucR family transcriptional regulator	-
CD178_RS17375	20493..20699	+	207	WP_118963922.1	Hin recombinase	-
CD178_RS17380	20919..21200	-	282	WP_118963923.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CD178_RS17385	21197..21484	-	288	WP_118963924.1	ribbon-helix-helix domain-containing protein	Antitoxin
CD178_RS17390	22539..23444	-	906	WP_118963925.1	replication protein RepA	-
CD178_RS17395	23646..23900	-	255	WP_081472694.1	ribbon-helix-helix domain-containing protein	-
CD178_RS17400	23930..24580	-	651	WP_118963926.1	AAA family ATPase	-
CD178_RS17405	24717..25352	-	636	WP_118963927.1	tyrosine-type recombinase/integrase	-
CD178_RS17410	25349..25645	-	297	WP_118963928.1	WGR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26645	26645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-48)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10801.30 Da
• Isoelectric Point: 6.7241

>T80362 WP_118963923.1 NZ_CP023041:c21200-20919 [Komagataeibacter saccharivorans]
VKYEWTAKAVSDLQTLTADFDHPSTAARVMRRLTAAPDVLIQFPRMGTLLPVFSPREVRRFIVDDYEIRYEVTADALFIT
DVFHTRQDRGTFH

Nucleotide

• Length: 282 bp

>T80362 NZ_CP023041:c21200-20919 [Komagataeibacter saccharivorans]
GTGAAATATGAATGGACGGCCAAGGCCGTATCTGATCTGCAAACGCTAACCGCAGATTTTGACCACCCGAGCACCGCCGC
GCGTGTTATGCGGCGGCTGACGGCAGCCCCGGACGTTCTGATCCAGTTCCCTCGGATGGGTACGCTGCTTCCGGTCTTCT
CACCCCGTGAGGTCCGGCGCTTCATCGTGGATGACTATGAAATTCGCTATGAGGTCACGGCGGATGCCCTGTTCATCACC
GACGTTTTCCATACGAGGCAGGACCGTGGAACGTTTCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10488.88 Da
• Isoelectric Point: 4.8660

>AT80362 WP_118963924.1 NZ_CP023041:c21484-21197 [Komagataeibacter saccharivorans]
MSEKLSAGKQVVSVQMPADLVDAMDRYGEAIERPRSWVMREAVASYLAYEAEKDRATRRALADVDAGRTISAEAVKAWAD
SLDQANPLPLPEPKR

Nucleotide

• Length: 288 bp

>AT80362 NZ_CP023041:c21484-21197 [Komagataeibacter saccharivorans]
ATGAGCGAGAAACTTTCAGCCGGAAAGCAGGTGGTCAGTGTGCAGATGCCTGCCGACCTGGTGGATGCGATGGATCGGTA
TGGGGAAGCCATCGAGCGGCCCAGGTCATGGGTGATGCGGGAAGCCGTGGCGTCTTACCTTGCCTATGAGGCAGAAAAAG
ACCGGGCGACGCGCCGTGCCCTGGCCGATGTAGATGCTGGCCGGACGATCAGTGCCGAGGCCGTCAAGGCATGGGCAGAC
AGTCTCGACCAGGCTAACCCGCTGCCGCTGCCGGAACCCAAGCGGTGA