Detailed information of TA system TA080359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 90247..90920
• Replicon: plasmid unnamed3
• Accession: NZ_CP023039
• Organism: Komagataeibacter saccharivorans strain CV1

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: -
• Locus tag: CD178_RS16870
• Protein ID: WP_110548355.1
• Coordinates: 90504..90920 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CD178_RS16865
• Protein ID: WP_174234184.1
• Coordinates: 90247..90507 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD178_RS16835	85851..86096	-	246	WP_110548360.1	hypothetical protein	-
CD178_RS16840	86107..87630	-	1524	WP_110548367.1	DNA polymerase Y family protein	-
CD178_RS16845	87521..88321	-	801	WP_110548359.1	damage-inducible mutagenesis protein	-
CD178_RS16850	88393..89043	-	651	WP_174234183.1	MucR family transcriptional regulator	-
CD178_RS16855	89436..89825	-	390	WP_110548357.1	hypothetical protein	-
CD178_RS16860	89818..90021	-	204	WP_110548356.1	hypothetical protein	-
CD178_RS16865	90247..90507	+	261	WP_174234184.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CD178_RS16870	90504..90920	+	417	WP_110548355.1	type II toxin-antitoxin system VapC family toxin	Toxin
CD178_RS16875	90966..92186	-	1221	WP_118963867.1	transposase	-
CD178_RS16880	92209..92622	+	414	WP_110548459.1	IS200/IS605 family transposase	-
CD178_RS16885	92981..93391	-	411	WP_110548428.1	type II toxin-antitoxin system VapC family toxin	-
CD178_RS16890	93388..93657	-	270	WP_110548427.1	antitoxin	-
CD178_RS16895	93961..94638	+	678	WP_110548426.1	hypothetical protein	-
CD178_RS16900	94706..95389	+	684	WP_110548425.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..127836	127836
	-	flank	IS/Tn	-	-	92209..92622	413

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-129)

Antitoxin

• SpoVT-AbrB domain (10-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.43 Da
• Isoelectric Point: 7.3852

>T80359 WP_110548355.1 NZ_CP023039:90504-90920 [Komagataeibacter saccharivorans]
MSADLTMSCMLDTNTVSHILKGHPRALERLSQVPMANVCISVITEGELRFGISKRPQAIRLQRIVSEFLQRVAVEPWESS
TAHAYGALRASLETSGKVVGALDLLIAAHALDLNATLVTNDAGFRKIAHLRTEDWTAY

Nucleotide

• Length: 417 bp

>T80359 NZ_CP023039:90504-90920 [Komagataeibacter saccharivorans]
ATGAGCGCAGATCTAACAATGTCCTGTATGCTTGACACCAACACGGTGAGTCACATTCTCAAGGGGCATCCCCGAGCATT
GGAACGCCTTTCTCAGGTACCTATGGCAAACGTGTGCATCTCCGTCATCACGGAAGGAGAATTGCGTTTCGGCATTTCCA
AGCGCCCGCAGGCGATACGGCTCCAGCGGATCGTGAGCGAGTTTCTCCAGCGTGTGGCTGTTGAACCATGGGAATCATCT
ACGGCCCACGCATATGGCGCCCTTCGCGCTTCTCTGGAAACCTCAGGCAAAGTGGTAGGTGCCCTTGACCTGCTCATCGC
TGCTCACGCCCTGGACCTGAATGCCACACTTGTTACCAATGATGCAGGCTTTCGCAAGATCGCCCATTTGAGGACCGAAG
ACTGGACAGCATACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9644.70 Da
• Isoelectric Point: 4.6050

>AT80359 WP_174234184.1 NZ_CP023039:90247-90507 [Komagataeibacter saccharivorans]
MTQVAKIFNNGRSQAVRLPAAFRFEGTEVFIRRDETSGDVILSRRPESWDGLLAAIREGGAPDDFLNTSERAQMQDDRDP
FADVSK

Nucleotide

• Length: 261 bp

>AT80359 NZ_CP023039:90247-90507 [Komagataeibacter saccharivorans]
ATGACGCAGGTCGCAAAAATCTTCAATAATGGCCGAAGCCAGGCGGTTCGCCTGCCGGCCGCTTTTCGCTTCGAAGGCAC
GGAAGTCTTTATTCGTCGGGACGAGACTTCCGGAGACGTCATCCTGTCGCGCCGACCGGAAAGTTGGGACGGTCTCCTTG
CCGCGATCAGGGAAGGTGGCGCGCCGGATGATTTCCTCAATACTTCTGAACGCGCACAGATGCAGGATGATCGCGACCCG
TTCGCCGACGTGTCGAAATGA