Detailed information of TA system TA080357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 23765..24400
• Replicon: plasmid unnamed2
• Accession: NZ_CP023038
• Organism: Komagataeibacter saccharivorans strain CV1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CD178_RS15715
• Protein ID: WP_010511772.1
• Coordinates: 23765..24175 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G2I1D7
• Locus tag: CD178_RS15720
• Protein ID: WP_010511771.1
• Coordinates: 24176..24400 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD178_RS15680	19354..19836	-	483	WP_010511786.1	Hsp20 family protein	-
CD178_RS15685	19872..20153	-	282	WP_010511784.1	hypothetical protein	-
CD178_RS15690	20152..20415	+	264	WP_102324447.1	YXWGXW repeat-containing protein	-
CD178_RS15695	20936..21184	+	249	WP_010511782.1	hypothetical protein	-
CD178_RS15700	21293..21556	+	264	WP_010511780.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
CD178_RS15705	21549..21893	+	345	WP_010511776.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CD178_RS15710	21929..23704	+	1776	WP_010511773.1	recombinase family protein	-
CD178_RS15715	23765..24175	-	411	WP_010511772.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CD178_RS15720	24176..24400	-	225	WP_010511771.1	hypothetical protein	Antitoxin
CD178_RS15725	24673..25005	+	333	WP_010511770.1	recombinase family protein	-
CD178_RS15730	25452..26066	+	615	WP_010511769.1	hydroxyethylthiazole kinase	-
CD178_RS15735	26119..26915	+	797	Protein_31	IS5 family transposase	-
CD178_RS15740	26905..28158	-	1254	WP_162900555.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..159308	159308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (9-55)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15368.59 Da
• Isoelectric Point: 6.2333

>T80357 WP_010511772.1 NZ_CP023038:c24175-23765 [Komagataeibacter saccharivorans]
MLRYLLDTNLCIRVLRDRPQGLRPRFNSCAEELCISDVVLYELLYGAERSSDPVGTRRDVEHFAARLAVLPFDSEAAAHT
ADIRAVLERNGRIIGPYDLMIAGHARSKGLIVVTGNLREFQRVEGLRTEDWLTDTV

Nucleotide

• Length: 411 bp

>T80357 NZ_CP023038:c24175-23765 [Komagataeibacter saccharivorans]
ATGCTGCGTTACCTGCTGGATACGAACCTCTGTATCCGGGTGTTGCGGGATCGGCCACAGGGGTTGCGTCCGAGGTTCAA
CAGCTGTGCGGAGGAACTCTGCATCTCGGACGTGGTTCTGTATGAGCTGCTTTATGGCGCGGAACGCTCGTCCGATCCCG
TAGGCACAAGACGCGACGTTGAGCACTTCGCGGCAAGGCTGGCGGTCCTGCCATTCGACAGTGAAGCGGCTGCCCATACG
GCGGACATTCGGGCTGTTCTTGAACGGAATGGCCGTATCATAGGTCCGTATGATCTCATGATTGCCGGGCATGCCCGGAG
CAAAGGGCTGATTGTGGTGACCGGCAATCTGCGGGAATTTCAGCGGGTGGAGGGTCTTCGAACGGAGGACTGGCTGACTG
ACACGGTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8217.39 Da
• Isoelectric Point: 4.9816

>AT80357 WP_010511771.1 NZ_CP023038:c24400-24176 [Komagataeibacter saccharivorans]
MLTRTTLFLSNRSQAVRLPKMVAFGEQVRDVVIVSEGSRRIIAPADAAWDDFFAAPGVDLGERNQPAMQEREAL

Nucleotide

• Length: 225 bp

>AT80357 NZ_CP023038:c24400-24176 [Komagataeibacter saccharivorans]
ATGCTGACCCGTACGACACTCTTTCTATCCAACCGCTCACAGGCGGTGCGTCTGCCGAAGATGGTTGCCTTCGGGGAGCA
GGTGCGTGACGTCGTCATCGTGTCCGAAGGGTCGCGGCGGATCATTGCTCCGGCCGATGCGGCGTGGGATGATTTCTTTG
CGGCTCCTGGGGTGGATCTGGGGGAGCGAAACCAGCCGGCCATGCAGGAACGTGAGGCGCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A094ZE42