Detailed information of TA system TA080356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 21293..21893
• Replicon: plasmid unnamed2
• Accession: NZ_CP023038
• Organism: Komagataeibacter saccharivorans strain CV1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A318QKN2
• Locus tag: CD178_RS15705
• Protein ID: WP_010511776.1
• Coordinates: 21549..21893 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5YEQ9
• Locus tag: CD178_RS15700
• Protein ID: WP_010511780.1
• Coordinates: 21293..21556 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD178_RS15650	16664..16933	+	270	WP_081472672.1	hypothetical protein	-
CD178_RS15655	17009..17641	+	633	WP_010511791.1	hypothetical protein	-
CD178_RS15660	17638..18015	+	378	WP_110560966.1	hypothetical protein	-
CD178_RS15665	18061..18249	-	189	WP_010511790.1	hypothetical protein	-
CD178_RS15670	18331..18888	+	558	WP_029335933.1	phosphate-starvation-inducible PsiE family protein	-
CD178_RS15680	19354..19836	-	483	WP_010511786.1	Hsp20 family protein	-
CD178_RS15685	19872..20153	-	282	WP_010511784.1	hypothetical protein	-
CD178_RS15690	20152..20415	+	264	WP_102324447.1	YXWGXW repeat-containing protein	-
CD178_RS15695	20936..21184	+	249	WP_010511782.1	hypothetical protein	-
CD178_RS15700	21293..21556	+	264	WP_010511780.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CD178_RS15705	21549..21893	+	345	WP_010511776.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CD178_RS15710	21929..23704	+	1776	WP_010511773.1	recombinase family protein	-
CD178_RS15715	23765..24175	-	411	WP_010511772.1	tRNA(fMet)-specific endonuclease VapC	-
CD178_RS15720	24176..24400	-	225	WP_010511771.1	hypothetical protein	-
CD178_RS15725	24673..25005	+	333	WP_010511770.1	recombinase family protein	-
CD178_RS15730	25452..26066	+	615	WP_010511769.1	hydroxyethylthiazole kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..159308	159308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13238.09 Da
• Isoelectric Point: 9.9064

>T80356 WP_010511776.1 NZ_CP023038:21549-21893 [Komagataeibacter saccharivorans]
MTEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNSHSPGNELHGDLAGFYKIKLRQDGYRLVYQVVEQRIVIFVIAV
GRREDSEIYTTATGRIPETPADRIKQPSGKSRKR

Nucleotide

• Length: 345 bp

>T80356 NZ_CP023038:21549-21893 [Komagataeibacter saccharivorans]
ATGACTGAATACGCGATCGAATTCGATGATCGCGCACTTCGGGAATGGGACGGGCTTGACGGCAGCATTCGCAGGAAGTT
CGAGAAAAAGCTCGAAAAACTGGTTCTGAACTCGCATTCTCCCGGTAACGAACTGCACGGCGATCTCGCGGGATTCTACA
AGATCAAACTGCGCCAGGACGGCTACCGCCTCGTCTATCAGGTCGTGGAACAGCGGATCGTCATTTTTGTCATCGCGGTC
GGACGCCGTGAGGACAGCGAAATCTACACGACGGCAACCGGGCGCATCCCGGAGACGCCAGCCGACCGGATAAAACAGCC
CAGCGGCAAAAGTCGCAAAAGGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9629.04 Da
• Isoelectric Point: 7.3200

>AT80356 WP_010511780.1 NZ_CP023038:21293-21556 [Komagataeibacter saccharivorans]
MSAVTFRVDDALKSAAVAKLSAHGLSLSDVLRDTLAYIAETGQPPVKRRLVTDEDARLIEIVRERLADPAPRHRMTLAEL
KARHPDD

Nucleotide

• Length: 264 bp

>AT80356 NZ_CP023038:21293-21556 [Komagataeibacter saccharivorans]
ATGTCCGCCGTGACGTTCCGTGTCGATGACGCCCTCAAATCCGCCGCTGTCGCCAAACTCTCCGCGCACGGCCTGTCTCT
CTCCGACGTCCTGCGCGATACCCTGGCCTATATCGCCGAGACCGGCCAGCCACCGGTCAAGCGGCGGCTTGTGACCGACG
AAGATGCAAGGCTGATCGAAATCGTCCGAGAACGCCTCGCCGATCCCGCCCCCCGCCACCGCATGACGCTGGCGGAACTC
AAGGCCCGCCATCCGGATGACTGA

Structures

Toxin

• AlphaFold DB: A0A318QKN2

Antitoxin

• AlphaFold DB: A0A7W4J448