Detailed information of TA system TA080355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2207837..2208420
• Replicon: chromosome
• Accession: NZ_CP023036
• Organism: Komagataeibacter saccharivorans strain CV1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W5Y5C8
• Locus tag: CD178_RS10275
• Protein ID: WP_019087476.1
• Coordinates: 2208166..2208420 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CD178_RS10270
• Protein ID: WP_118963056.1
• Coordinates: 2207837..2208169 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD178_RS10215	2202862..2203653	+	792	WP_118963050.1	phage regulatory protein/antirepressor Ant	-
CD178_RS10220	2203884..2204150	+	267	WP_118963637.1	hypothetical protein	-
CD178_RS10225	2204200..2204499	-	300	WP_174234107.1	HigA family addiction module antidote protein	-
CD178_RS10230	2204489..2204779	-	291	WP_118963051.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CD178_RS10235	2204939..2205232	+	294	WP_118963052.1	hypothetical protein	-
CD178_RS10240	2205232..2205606	+	375	WP_039733460.1	hypothetical protein	-
CD178_RS10245	2205606..2206028	+	423	WP_118963639.1	cell wall hydrolase	-
CD178_RS10250	2206025..2206372	+	348	WP_118963053.1	hypothetical protein	-
CD178_RS10255	2206359..2206694	+	336	WP_118963054.1	hypothetical protein	-
CD178_RS10260	2206705..2207061	+	357	WP_118963055.1	hypothetical protein	-
CD178_RS10265	2207070..2207270	+	201	WP_118963640.1	hypothetical protein	-
CD178_RS10270	2207837..2208169	-	333	WP_118963056.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CD178_RS10275	2208166..2208420	-	255	WP_019087476.1	type II toxin-antitoxin system HicA family toxin	Toxin
CD178_RS10280	2209070..2210431	-	1362	WP_118963057.1	8-oxoguanine deaminase	-
CD178_RS10285	2210916..2211650	+	735	WP_118963058.1	aspartyl/asparaginyl beta-hydroxylase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9431.88 Da
• Isoelectric Point: 10.7588

>T80355 WP_019087476.1 NZ_CP023036:c2208420-2208166 [Komagataeibacter saccharivorans]
MKAKHRRTLELIFKRPVSGNVPWSDIEALFVSLGAEVSEREGSRIGVVLFGEIRVFHRPHPKPDTDKGAVASVRKWLEQN
GVTP

Nucleotide

• Length: 255 bp

>T80355 NZ_CP023036:c2208420-2208166 [Komagataeibacter saccharivorans]
ATGAAAGCCAAGCACCGTCGCACCCTTGAACTGATCTTCAAACGCCCCGTTTCCGGCAACGTGCCATGGAGCGACATTGA
GGCCCTGTTCGTCTCGCTAGGCGCCGAAGTTTCGGAACGGGAAGGCTCACGCATTGGCGTAGTCCTGTTCGGGGAGATCA
GGGTTTTTCATCGCCCTCACCCCAAACCGGATACGGATAAGGGAGCGGTGGCATCGGTGCGGAAATGGCTGGAGCAGAAC
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12129.86 Da
• Isoelectric Point: 5.1784

>AT80355 WP_118963056.1 NZ_CP023036:c2208169-2207837 [Komagataeibacter saccharivorans]
MNNMMEIGSHRAVIQFDPEIGMFRGEFLGLNGGADFYADSVEGLRTEGEISLRVFLDMCEAKGIAPTKRYSGKFQVRLSP
DVHARAAEIAAARGVSLNRLVQDALELVER

Nucleotide

• Length: 333 bp

>AT80355 NZ_CP023036:c2208169-2207837 [Komagataeibacter saccharivorans]
ATGAATAACATGATGGAGATTGGCAGCCACCGGGCTGTCATCCAGTTTGACCCGGAAATCGGCATGTTCCGGGGCGAGTT
CCTTGGCCTGAACGGCGGGGCTGACTTTTACGCGGACAGCGTGGAAGGGCTGCGTACGGAAGGCGAGATCTCGCTGCGGG
TGTTTCTGGATATGTGCGAGGCAAAGGGAATCGCCCCCACGAAACGCTACTCCGGCAAGTTTCAGGTCCGCCTGTCCCCG
GATGTTCATGCCCGCGCCGCTGAGATCGCAGCCGCCCGTGGCGTGAGCCTGAACCGACTGGTTCAGGACGCGTTGGAGCT
TGTGGAGCGGTAA

Structures

Toxin

• AlphaFold DB: W5Y5C8