Detailed information of TA system TA080331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 273302..273873
• Replicon: chromosome
• Accession: NZ_CP023011
• Organism: Enterococcus hirae strain FDAARGOS_234

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A6J73_RS01300
• Protein ID: WP_094940837.1
• Coordinates: 273532..273873 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A6J73_RS01295
• Protein ID: WP_094940838.1
• Coordinates: 273302..273532 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J73_RS01285	270308..271273	+	966	WP_081127653.1	helix-turn-helix domain-containing protein	-
A6J73_RS01290	271454..272833	-	1380	WP_010738534.1	FAD-dependent oxidoreductase	-
A6J73_RS01295	273302..273532	+	231	WP_094940838.1	AbrB family transcriptional regulator	Antitoxin
A6J73_RS01300	273532..273873	+	342	WP_094940837.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A6J73_RS01305	274037..275203	-	1167	WP_094940836.1	pyridoxal phosphate-dependent aminotransferase	-
A6J73_RS01310	275233..276342	-	1110	WP_164445114.1	amidohydrolase	-
A6J73_RS01315	276302..277651	-	1350	WP_053765847.1	aspartate kinase	-
A6J73_RS01320	277648..278649	-	1002	WP_094940834.1	aspartate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13128.52 Da
• Isoelectric Point: 10.1266

>T80331 WP_094940837.1 NZ_CP023011:273532-273873 [Enterococcus hirae]
VPKVRNYIPKKGDIVWIDFDPSTGKGIQKRRLGLFVSCYEFNCTTMFAIIYPITSTIKNLPTRYSLPKDLDTKGQVLISQ
LKSLDFKERKLKKVENLPLQDMAKIDQIIQYIF

Nucleotide

• Length: 342 bp

>T80331 NZ_CP023011:273532-273873 [Enterococcus hirae]
GTGCCTAAAGTTAGAAATTATATTCCTAAAAAAGGGGATATTGTTTGGATCGATTTTGATCCATCCACAGGCAAAGGAAT
TCAAAAGAGAAGACTAGGATTGTTTGTATCTTGTTATGAATTTAATTGCACAACAATGTTTGCAATAATCTATCCAATTA
CTTCTACTATAAAAAATCTACCGACACGCTATTCGCTTCCCAAAGATTTAGATACAAAGGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTCAAGGAAAGAAAACTCAAAAAGGTCGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8442.43 Da
• Isoelectric Point: 4.0078

>AT80331 WP_094940838.1 NZ_CP023011:273302-273532 [Enterococcus hirae]
VLIAKSILQGGSVVITLPSDNGKKPSENQEYIVVYSEDGTITLVPKIEDPFSAGQEAEYYEKDEWKDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT80331 NZ_CP023011:273302-273532 [Enterococcus hirae]
GTGCTCATTGCAAAATCTATATTACAAGGTGGTTCTGTAGTAATTACTCTACCATCTGATAATGGTAAAAAACCTTCAGA
AAATCAAGAATATATTGTTGTCTACTCTGAAGATGGGACAATCACATTAGTTCCTAAAATCGAAGATCCATTTAGTGCTG
GACAAGAAGCTGAGTATTACGAAAAAGATGAATGGAAAGACTTAACTCCTGAAGGGAGAGAAATTTTATAG