Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2149021..2149646 Replicon chromosome
Accession NZ_CP023009
Organism Lonsdalea britannica strain 477

Toxin (Protein)


Gene name Hha Uniprot ID E0SD87
Locus tag CKQ53_RS09865 Protein ID WP_009114000.1
Coordinates 2149021..2149224 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1X3RNN7
Locus tag CKQ53_RS09870 Protein ID WP_026738849.1
Coordinates 2149278..2149646 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKQ53_RS09835 2144638..2144976 + 339 WP_009114005.1 P-II family nitrogen regulator -
CKQ53_RS09840 2145016..2146308 + 1293 WP_085652948.1 ammonium transporter AmtB -
CKQ53_RS09845 2146412..2147275 - 864 WP_085652972.1 acyl-CoA thioesterase II -
CKQ53_RS09850 2147483..2148055 + 573 WP_085652949.1 YbaY family lipoprotein -
CKQ53_RS09855 2148087..2148407 - 321 WP_094118523.1 MGMT family protein -
CKQ53_RS09865 2149021..2149224 - 204 WP_009114000.1 hemolysin expression modulator Hha Toxin
CKQ53_RS09870 2149278..2149646 - 369 WP_026738849.1 Hha toxicity modulator TomB Antitoxin
CKQ53_RS09875 2150271..2150414 - 144 WP_085652951.1 type B 50S ribosomal protein L36 -
CKQ53_RS09880 2150433..2150684 - 252 WP_085652952.1 type B 50S ribosomal protein L31 -
CKQ53_RS09885 2150828..2153974 - 3147 WP_118894947.1 efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8133.51 Da        Isoelectric Point: 8.8500

>T80329 WP_009114000.1 NZ_CP023009:c2149224-2149021 [Lonsdalea britannica]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Download         Length: 204 bp

>T80329 NZ_CP023009:c2149224-2149021 [Lonsdalea britannica]
ATGAAAAAAATAGATTACTTGATGCGTTTGCGCAAATGCACAACCATCGACACCCTTGAACGAGTTATTGAAAAAAACAA
GTATGAACTCTCTAACGATGAGTTAGAAATGTTCTATTCCGCGGCAGACCACCGTCTGGCCGAGCTAACCATGAATAAGC
TTTACGACAAAGTCCCTACTGCCGTATGGAAATACGTACGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14381.12 Da        Isoelectric Point: 4.7628

>AT80329 WP_026738849.1 NZ_CP023009:c2149646-2149278 [Lonsdalea britannica]
MDEYSPKHYDIAQLRFLCENLRDESIATLGDSNHGWVNDPTSAINLQLNELIEHIAAFIGTYKIKYPDDEELYMRVEKYL
DDTYILFSNYGINDAELRRWQKSKSQLFRMLSEKSSYATVRT

Download         Length: 369 bp

>AT80329 NZ_CP023009:c2149646-2149278 [Lonsdalea britannica]
ATGGATGAGTACTCACCTAAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAGAATCTGCGCGACGAAAGTATCGC
GACACTAGGTGACAGCAACCACGGCTGGGTGAATGATCCGACCTCCGCAATAAATCTTCAGCTCAATGAACTTATTGAGC
ATATTGCGGCCTTTATTGGCACCTATAAAATTAAATACCCGGATGACGAAGAGTTGTACATGCGGGTTGAAAAATATTTA
GACGATACTTATATTCTTTTCAGCAATTACGGCATTAATGATGCGGAGCTGCGGAGATGGCAAAAATCTAAGTCACAATT
ATTCAGAATGCTCTCAGAAAAGAGCAGCTATGCAACAGTAAGAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X3RNQ0

Antitoxin

Source ID Structure
AlphaFold DB A0A1X3RNN7

References