Detailed information of TA system TA080317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1780526..1781088
• Replicon: chromosome
• Accession: NZ_CP022998
• Organism: Rhizobium sp. 11515TR strain 10195

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CKA34_RS08695
• Protein ID: WP_095434323.1
• Coordinates: 1780526..1780894 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1E4Y0V5
• Locus tag: CKA34_RS08700
• Protein ID: WP_069616104.1
• Coordinates: 1780891..1781088 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKA34_RS08675	1775587..1776819	-	1233	WP_095434319.1	efflux RND transporter periplasmic adaptor subunit	-
CKA34_RS08680	1777245..1778033	-	789	WP_095434320.1	SDR family oxidoreductase	-
CKA34_RS08685	1778326..1779990	+	1665	WP_095434321.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
CKA34_RS08690	1779997..1780524	+	528	WP_095434322.1	GNAT family N-acetyltransferase	-
CKA34_RS08695	1780526..1780894	-	369	WP_095434323.1	type II toxin-antitoxin system VapC family toxin	Toxin
CKA34_RS08700	1780891..1781088	-	198	WP_069616104.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CKA34_RS08705	1781191..1782093	-	903	WP_095436216.1	DMT family transporter	-
CKA34_RS08710	1782238..1782711	+	474	WP_095434324.1	Lrp/AsnC family transcriptional regulator	-
CKA34_RS08715	1782778..1784151	-	1374	WP_095434325.1	magnesium transporter	-
CKA34_RS08720	1784506..1785519	-	1014	WP_095434326.1	zinc-binding alcohol dehydrogenase family protein	-
CKA34_RS08725	1785624..1786028	+	405	WP_095434327.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-47)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13712.96 Da
• Isoelectric Point: 9.5051

>T80317 WP_095434323.1 NZ_CP022998:c1780894-1780526 [Rhizobium sp. 11515TR]
MILVDTSIWIDHFHKPEARLQSLLEQKQVVVHPFVVGEIGLGNLRQYDLIMGSLRRLPPIARTSDEDVLYFIRSNKLQGA
GIGYIDAHLAAATILAPGTYLWTRDKRRLQRVAENLGIAAPL

Nucleotide

• Length: 369 bp

>T80317 NZ_CP022998:c1780894-1780526 [Rhizobium sp. 11515TR]
ATGATCCTGGTCGATACGTCGATCTGGATCGATCACTTTCATAAGCCGGAGGCGCGGCTCCAATCTCTGCTGGAGCAAAA
GCAGGTCGTCGTTCATCCTTTCGTCGTCGGTGAGATCGGCCTTGGAAATCTGCGGCAATATGATCTCATAATGGGAAGCC
TCCGCCGACTGCCCCCGATTGCGAGAACCTCGGATGAGGACGTGCTTTATTTCATCCGAAGCAACAAACTGCAGGGTGCG
GGAATCGGCTATATCGATGCTCATCTCGCGGCCGCGACAATACTGGCGCCTGGAACCTATCTCTGGACGCGAGACAAGCG
GCGGCTGCAACGTGTCGCCGAAAACCTCGGAATTGCCGCGCCGCTATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7045.13 Da
• Isoelectric Point: 6.2935

>AT80317 WP_069616104.1 NZ_CP022998:c1781088-1780891 [Rhizobium sp. 11515TR]
MRTTVAIDDKLLADAREVTGLAETSAILREALKALVEREAAKTLIRLGGTEPNITAPPRRQFGVE

Nucleotide

• Length: 198 bp

>AT80317 NZ_CP022998:c1781088-1780891 [Rhizobium sp. 11515TR]
ATGCGCACCACCGTAGCCATCGACGATAAGTTGCTTGCGGATGCACGCGAGGTTACCGGTCTCGCCGAGACCTCGGCGAT
CCTGCGTGAAGCGTTGAAAGCGCTTGTGGAACGGGAAGCCGCCAAAACCCTGATACGTCTCGGCGGCACCGAACCGAATA
TCACAGCTCCCCCTCGTCGCCAGTTTGGCGTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1E4Y0V5