Detailed information of TA system TA080295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-UPF0150
• Location: 4300376..4301026
• Replicon: chromosome
• Accession: NZ_CP022989
• Organism: Paraburkholderia aromaticivorans strain BN5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CJU94_RS19365
• Protein ID: WP_095420439.1
• Coordinates: 4300376..4300552 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CJU94_RS19370
• Protein ID: WP_095420074.1
• Coordinates: 4300610..4301026 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU94_RS19340	4295591..4296721	+	1131	WP_095420069.1	IS110 family transposase	-
CJU94_RS19345	4297057..4298043	+	987	WP_167397546.1	winged helix-turn-helix domain-containing protein	-
CJU94_RS19350	4298173..4298526	+	354	WP_095420071.1	DUF2591 family protein	-
CJU94_RS19355	4298495..4299670	-	1176	WP_157763775.1	acyltransferase	-
CJU94_RS19360	4299751..4300208	-	458	Protein_3791	SOS response-associated peptidase family protein	-
CJU94_RS19365	4300376..4300552	+	177	WP_095420439.1	type II toxin-antitoxin system HicA family toxin	Toxin
CJU94_RS19370	4300610..4301026	+	417	WP_095420074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CJU94_RS19375	4301100..4301489	-	390	WP_208645328.1	DUF1353 domain-containing protein	-
CJU94_RS41580	4301486..4301983	-	498	WP_208645329.1	hypothetical protein	-
CJU94_RS19380	4302024..4302242	-	219	WP_095420076.1	hypothetical protein	-
CJU94_RS19385	4302251..4302736	-	486	WP_095420077.1	hypothetical protein	-
CJU94_RS19390	4302793..4303365	-	573	WP_095420078.1	chitinase	-
CJU94_RS19395	4303362..4303691	-	330	WP_157763776.1	hypothetical protein	-
CJU94_RS19400	4303818..4305218	-	1401	WP_095417514.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4285582..4347898	62316
	-	flank	IS/Tn	-	-	4295591..4296721	1130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6831.89 Da
• Isoelectric Point: 10.6867

>T80295 WP_095420439.1 NZ_CP022989:4300376-4300552 [Paraburkholderia aromaticivorans]
VKQEEFKRWLEEQGVVVENGKKHWKAYYNGKQTTVPRHPGKELKDGTRRAILKQLGLK

Nucleotide

• Length: 177 bp

>T80295 NZ_CP022989:4300376-4300552 [Paraburkholderia aromaticivorans]
GTGAAACAGGAAGAGTTTAAGCGGTGGTTGGAAGAGCAAGGCGTGGTAGTAGAGAACGGCAAGAAGCACTGGAAGGCCTA
CTACAACGGAAAGCAGACCACCGTACCGAGGCACCCCGGCAAGGAACTGAAGGATGGAACCAGGCGGGCGATTCTCAAAC
AACTTGGACTGAAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15053.34 Da
• Isoelectric Point: 4.6584

>AT80295 WP_095420074.1 NZ_CP022989:4300610-4301026 [Paraburkholderia aromaticivorans]
MLSYPAVFEPDSGGFVVTFRDIPEAITQGDSLEEARSMAADALLTAMDFYFEDKRPVPAPSKAKKGEELVALPASASAKI
LLLNEMLAQKVTPSELARRLDTRPQDVNRIVNLEHTTKIDTIAEALAALGKRLEITVI

Nucleotide

• Length: 417 bp

>AT80295 NZ_CP022989:4300610-4301026 [Paraburkholderia aromaticivorans]
ATGTTGTCTTATCCGGCTGTATTTGAGCCTGACAGCGGCGGGTTCGTCGTTACGTTCCGGGATATTCCGGAGGCAATCAC
TCAGGGCGATTCCCTCGAAGAGGCTCGCTCTATGGCTGCTGATGCATTGCTCACGGCGATGGACTTTTATTTTGAGGACA
AGCGCCCAGTTCCTGCGCCGTCGAAAGCGAAGAAGGGAGAGGAGTTGGTCGCGCTGCCCGCCAGCGCGTCAGCGAAGATC
CTGCTGCTCAACGAGATGCTGGCGCAGAAGGTCACGCCGTCGGAGCTTGCCCGACGGCTGGATACGCGTCCTCAGGATGT
GAATCGCATCGTCAACCTCGAACACACTACGAAGATTGACACGATTGCCGAGGCGCTGGCAGCACTCGGAAAGCGCCTAG
AGATCACTGTTATTTAA