Detailed information of TA system TA080292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2747063..2747592
• Replicon: chromosome
• Accession: NZ_CP022987
• Organism: Pusillimonas thiosulfatoxidans strain YE3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CKA81_RS13385
• Protein ID: WP_128355725.1
• Coordinates: 2747269..2747592 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CKA81_RS13380
• Protein ID: WP_128355724.1
• Coordinates: 2747063..2747272 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKA81_RS13360	2742909..2744312	+	1404	WP_128355721.1	DUF560 domain-containing protein	-
CKA81_RS13365	2744332..2744874	-	543	WP_128355722.1	transcriptional activator RfaH	-
CKA81_RS13370	2745063..2745649	+	587	Protein_2617	NYN domain-containing protein	-
CKA81_RS13375	2745706..2746764	+	1059	WP_128355723.1	dTDP-glucose 4,6-dehydratase	-
CKA81_RS13380	2747063..2747272	+	210	WP_128355724.1	antitoxin MazE family protein	Antitoxin
CKA81_RS13385	2747269..2747592	+	324	WP_128355725.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CKA81_RS13390	2747620..2747907	-	288	WP_128355726.1	hypothetical protein	-
CKA81_RS13395	2748080..2749021	-	942	WP_128355727.1	isoaspartyl peptidase/L-asparaginase	-
CKA81_RS13400	2749018..2749902	-	885	WP_128356802.1	DMT family transporter	-
CKA81_RS13405	2749975..2752581	-	2607	WP_128355728.1	alpha-ketoglutarate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (5-68)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11612.60 Da
• Isoelectric Point: 7.9849

>T80292 WP_128355725.1 NZ_CP022987:2747269-2747592 [Pusillimonas thiosulfatoxidans]
VTVRGDLVTVSLQGDYGKPRPALIIQSNLLAELESVIVCPITSELRNALFRITIEPDLSNGLQALSQVMADKIATIPRAK
IGKTFGRIDDERMRAVERALVLVTGIA

Nucleotide

• Length: 324 bp

>T80292 NZ_CP022987:2747269-2747592 [Pusillimonas thiosulfatoxidans]
GTGACTGTTCGTGGCGATCTAGTAACAGTGTCTTTACAAGGGGACTATGGCAAGCCTCGCCCGGCCTTAATCATCCAATC
CAATCTCTTGGCTGAGCTGGAAAGCGTAATCGTATGCCCGATTACCAGCGAGCTGCGTAACGCCCTCTTTCGCATCACGA
TAGAGCCGGACTTGAGCAATGGGCTGCAAGCCCTGTCTCAAGTCATGGCAGATAAGATTGCAACTATCCCTCGGGCGAAA
ATAGGCAAAACCTTCGGGCGGATAGACGACGAAAGAATGCGGGCGGTCGAGCGAGCGCTGGTGCTAGTAACCGGAATTGC
CTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7891.06 Da
• Isoelectric Point: 8.0582

>AT80292 WP_128355724.1 NZ_CP022987:2747063-2747272 [Pusillimonas thiosulfatoxidans]
MPTISKVERHRAKLRAAGLRPIQMWVPDTRIPEIAQQLQQQCRQLKNDPAEHDMLGFVEAAAAQTEGWQ

Nucleotide

• Length: 210 bp

>AT80292 NZ_CP022987:2747063-2747272 [Pusillimonas thiosulfatoxidans]
ATGCCGACGATAAGCAAAGTAGAGCGCCATCGCGCCAAGCTGCGCGCTGCAGGTCTGCGCCCAATTCAGATGTGGGTTCC
TGACACTCGCATACCCGAGATCGCGCAGCAGCTTCAGCAACAATGTCGCCAGCTAAAGAATGATCCAGCCGAGCACGACA
TGTTGGGGTTCGTCGAAGCGGCTGCTGCGCAAACTGAAGGCTGGCAGTGA