Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 1570084..1570720 Replicon chromosome
Accession NZ_CP022983
Organism Cytobacillus kochii strain BDGP4

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag CKF48_RS07920 Protein ID WP_095370824.1
Coordinates 1570084..1570434 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag CKF48_RS07925 Protein ID WP_095373546.1
Coordinates 1570439..1570720 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKF48_RS07885 1565447..1566241 - 795 WP_095370818.1 RNA polymerase sigma factor SigB -
CKF48_RS07890 1566219..1566692 - 474 WP_095370819.1 anti-sigma B factor RsbW -
CKF48_RS07895 1566689..1567021 - 333 WP_095370820.1 anti-sigma factor antagonist -
CKF48_RS07900 1567080..1568093 - 1014 WP_095370821.1 PP2C family protein-serine/threonine phosphatase -
CKF48_RS07905 1568106..1568507 - 402 WP_095370822.1 anti-sigma regulatory factor -
CKF48_RS07910 1568512..1568868 - 357 WP_066192954.1 STAS domain-containing protein -
CKF48_RS07915 1568873..1569691 - 819 WP_095370823.1 STAS domain-containing protein -
CKF48_RS07920 1570084..1570434 - 351 WP_095370824.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CKF48_RS07925 1570439..1570720 - 282 WP_095373546.1 antitoxin endoai Antitoxin
CKF48_RS07930 1570938..1572086 - 1149 WP_095370825.1 alanine racemase -
CKF48_RS07935 1572363..1573382 - 1020 WP_095370826.1 outer membrane lipoprotein carrier protein LolA -
CKF48_RS07940 1573613..1573969 - 357 WP_095370827.1 holo-ACP synthase -
CKF48_RS07945 1574061..1574786 + 726 WP_095370828.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13019.07 Da        Isoelectric Point: 5.1663

>T80284 WP_095370824.1 NZ_CP022983:c1570434-1570084 [Cytobacillus kochii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYAFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVNEALQVSLGLIEF

Download         Length: 351 bp

>T80284 NZ_CP022983:c1570434-1570084 [Cytobacillus kochii]
TTGATTGTTAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGCTCAGAACAAGGCGGTGTCCGTCCAGT
ACTTGTTATACAAAACGACATCGGGAATCGGTTTAGTCCCACAGTAATCATCGCAGCAATTACCGCTCAGATTCAAAAAG
CAAAGCTGCCTACTCACGTTGAAATTGATGCGAAGCGATATGCTTTTGAAAGAGATTCAGTAATATTGTTAGAACAAATT
CGTACAATTGATAAGCAGAGATTAACCGATAAAATCACCCATCTTGATGACGAAATGATGGATAAGGTAAATGAAGCCTT
ACAAGTCAGTTTAGGTCTTATAGAATTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10741.19 Da        Isoelectric Point: 7.2017

>AT80284 WP_095373546.1 NZ_CP022983:c1570720-1570439 [Cytobacillus kochii]
VSESSATTEIVVKLPQHLLTELDGYAKQENVNRSEFIYQATKMYLRERKKRHIRESMRRGYMEMAKLNLTIASEAFQAEY
EAENAVERLVSGG

Download         Length: 282 bp

>AT80284 NZ_CP022983:c1570720-1570439 [Cytobacillus kochii]
GTGTCTGAATCCAGCGCAACAACAGAAATAGTGGTGAAGCTGCCGCAACATCTATTAACGGAATTAGATGGTTATGCGAA
GCAAGAGAACGTAAACAGAAGTGAGTTTATTTATCAAGCAACGAAAATGTATTTGCGTGAACGGAAGAAAAGACATATTC
GTGAGTCCATGAGACGTGGTTACATGGAAATGGCAAAATTAAACTTGACGATAGCATCTGAAGCATTTCAAGCGGAATAC
GAGGCAGAAAACGCAGTTGAACGTCTTGTAAGCGGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References