Detailed information of TA system TA080279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 2938863..2939394
• Replicon: chromosome
• Accession: NZ_CP022962
• Organism: Bordetella bronchiseptica strain KM22

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: CJ015_RS13780
• Protein ID: WP_003812626.1
• Coordinates: 2938863..2939129 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: CJ015_RS13785
• Protein ID: WP_003812628.1
• Coordinates: 2939116..2939394 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJ015_RS13755	2933990..2934262	+	273	WP_003812615.1	hypothetical protein	-
CJ015_RS13760	2934301..2935011	-	711	WP_003812617.1	aquaporin Z	-
CJ015_RS13765	2935122..2935754	-	633	WP_003812619.1	PAS domain-containing protein	-
CJ015_RS13770	2936151..2937029	+	879	WP_003812621.1	tripartite tricarboxylate transporter substrate binding protein	-
CJ015_RS13775	2937091..2938632	+	1542	WP_003812624.1	M81 family metallopeptidase	-
CJ015_RS13780	2938863..2939129	+	267	WP_003812626.1	BrnT family toxin	Toxin
CJ015_RS13785	2939116..2939394	+	279	WP_003812628.1	BrnA antitoxin family protein	Antitoxin
CJ015_RS13795	2939688..2940344	-	657	WP_003812630.1	phosphoribosylanthranilate isomerase	-
CJ015_RS13800	2940359..2942024	-	1666	Protein_2726	TRAP transporter large permease subunit	-
CJ015_RS13805	2942027..2942656	-	630	WP_033451894.1	TRAP transporter small permease subunit	-
CJ015_RS13810	2942868..2943962	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T80279 WP_003812626.1 NZ_CP022962:2938863-2939129 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T80279 NZ_CP022962:2938863-2939129 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10426.92 Da
• Isoelectric Point: 10.5548

>AT80279 WP_003812628.1 NZ_CP022962:2939116-2939394 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQIVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT80279 NZ_CP022962:2939116-2939394 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGA
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61