Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4282086..4282583 Replicon chromosome
Accession NZ_CP022959
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CJZ69_RS21220 Protein ID WP_001173343.1
Coordinates 4282086..4282259 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CJZ69_RS21225 Protein ID WP_000398619.1
Coordinates 4282287..4282583 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJZ69_RS21210 4278756..4280213 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
CJZ69_RS21215 4280450..4281967 + 1518 WP_001295087.1 lysine--tRNA ligase -
CJZ69_RS21220 4282086..4282259 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CJZ69_RS21225 4282287..4282583 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CJZ69_RS21230 4282810..4283082 - 273 WP_000405647.1 N-acetyltransferase -
CJZ69_RS21235 4283094..4283324 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
CJZ69_RS21240 4283505..4285136 + 1632 WP_001216477.1 sensor histidine kinase -
CJZ69_RS21245 4285133..4285852 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4266607..4282583 15976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T80273 WP_001173343.1 NZ_CP022959:c4282259-4282086 [Escherichia coli ATCC 8739]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T80273 NZ_CP022959:c4282259-4282086 [Escherichia coli ATCC 8739]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT80273 WP_000398619.1 NZ_CP022959:c4282583-4282287 [Escherichia coli ATCC 8739]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT80273 NZ_CP022959:c4282583-4282287 [Escherichia coli ATCC 8739]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References