Detailed information of TA system TA080266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2441411..2442049
• Replicon: chromosome
• Accession: NZ_CP022959
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CJZ69_RS12075
• Protein ID: WP_000813794.1
• Coordinates: 2441873..2442049 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CJZ69_RS12070
• Protein ID: WP_001270286.1
• Coordinates: 2441411..2441827 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJZ69_RS12050	2436563..2437504	-	942	WP_001251313.1	ABC transporter permease	-
CJZ69_RS12055	2437505..2438518	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
CJZ69_RS12060	2438536..2439681	-	1146	WP_000047426.1	ABC transporter substrate-binding protein	-
CJZ69_RS12065	2439926..2441332	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
CJZ69_RS12070	2441411..2441827	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CJZ69_RS12075	2441873..2442049	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CJZ69_RS12080	2442271..2442501	+	231	WP_000494244.1	YncJ family protein	-
CJZ69_RS12085	2442593..2444554	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CJZ69_RS12090	2444627..2445163	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
CJZ69_RS12095	2445216..2446430	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T80266 WP_000813794.1 NZ_CP022959:c2442049-2441873 [Escherichia coli ATCC 8739]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T80266 NZ_CP022959:c2442049-2441873 [Escherichia coli ATCC 8739]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT80266 WP_001270286.1 NZ_CP022959:c2441827-2441411 [Escherichia coli ATCC 8739]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT80266 NZ_CP022959:c2441827-2441411 [Escherichia coli ATCC 8739]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB