80247

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2772085..2772736 Replicon chromosome
Accession NZ_CP022954
Organism Lacticaseibacillus paracasei subsp. paracasei strain IBB3423

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag LCAKO_RS13710 Protein ID WP_003567661.1
Coordinates 2772085..2772468 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A829TRS3
Locus tag LCAKO_RS13715 Protein ID WP_003606045.1
Coordinates 2772488..2772736 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCAKO_RS13705 2771380..2771901 - 522 WP_003567657.1 QueT transporter family protein -
LCAKO_RS13710 2772085..2772468 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LCAKO_RS13715 2772488..2772736 - 249 WP_003606045.1 programmed cell death antitoxin Antitoxin
LCAKO_RS13720 2772804..2773940 - 1137 WP_003606047.1 alanine racemase -
LCAKO_RS13725 2773927..2774301 - 375 WP_003567669.1 holo-ACP synthase -
LCAKO_RS13730 2774471..2775979 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
LCAKO_RS16140 2776105..2776248 - 144 WP_003591975.1 hypothetical protein -
LCAKO_RS13735 2776279..2777667 - 1389 WP_003606050.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T80247 WP_003567661.1 NZ_CP022954:c2772468-2772085 [Lacticaseibacillus paracasei subsp. paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T80247 NZ_CP022954:c2772468-2772085 [Lacticaseibacillus paracasei subsp. paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCAGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGCTTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8968.11 Da        Isoelectric Point: 4.4610

>AT80247 WP_003606045.1 NZ_CP022954:c2772736-2772488 [Lacticaseibacillus paracasei subsp. paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQTVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT80247 NZ_CP022954:c2772736-2772488 [Lacticaseibacillus paracasei subsp. paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGACTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A829TRS3

References