Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 171326..171969 Replicon plasmid pJYC01A
Accession NZ_CP022926
Organism Klebsiella pneumoniae strain ST307PT01

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CJ258_RS27590 Protein ID WP_001044770.1
Coordinates 171326..171742 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CJ258_RS27595 Protein ID WP_001261282.1
Coordinates 171739..171969 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ258_RS27555 166868..167104 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
CJ258_RS27560 167101..167463 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
CJ258_RS27565 167481..169175 - 1695 WP_000105636.1 mercury(II) reductase -
CJ258_RS27570 169227..169649 - 423 WP_001340589.1 organomercurial transporter MerC -
CJ258_RS27575 169685..169795 - 111 Protein_172 mercuric transport protein periplasmic component -
CJ258_RS27580 169811..170508 - 698 Protein_173 IS1 family transposase -
CJ258_RS27585 170542..171252 - 711 WP_004152334.1 ATP-binding protein -
CJ258_RS27590 171326..171742 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CJ258_RS27595 171739..171969 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CJ258_RS27600 171926..172387 + 462 WP_072093212.1 hypothetical protein -
CJ258_RS27610 172874..173182 + 309 WP_004152336.1 hypothetical protein -
CJ258_RS27615 173210..173539 + 330 WP_004152337.1 hypothetical protein -
CJ258_RS27620 173565..173963 + 399 WP_004171440.1 hypothetical protein -
CJ258_RS27625 173970..174302 + 333 WP_004152339.1 hypothetical protein -
CJ258_RS27630 174302..175084 + 783 WP_004152340.1 site-specific integrase -
CJ258_RS27635 175976..176206 - 231 WP_011977773.1 hypothetical protein -
CJ258_RS27640 176298..176771 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / blaOXA-1 / tet(A) / qnrB1 - 1..258210 258210
- flank IS/Tn - - 176891..178159 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T80242 WP_001044770.1 NZ_CP022926:c171742-171326 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T80242 NZ_CP022926:c171742-171326 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT80242 WP_001261282.1 NZ_CP022926:c171969-171739 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT80242 NZ_CP022926:c171969-171739 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References