Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 100006..100742 Replicon plasmid pJYC01A
Accession NZ_CP022926
Organism Klebsiella pneumoniae strain ST307PT01

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CJ258_RS27160 Protein ID WP_003026803.1
Coordinates 100260..100742 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CJ258_RS27155 Protein ID WP_003026799.1
Coordinates 100006..100272 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ258_RS27110 96068..96430 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
CJ258_RS27115 96480..96830 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CJ258_RS27120 97188..97457 + 270 WP_004152102.1 hypothetical protein -
CJ258_RS27125 97445..98020 + 576 WP_004152103.1 hypothetical protein -
CJ258_RS27130 98051..98545 + 495 WP_004152104.1 DNA-binding protein -
CJ258_RS27135 98589..98957 + 369 WP_004152105.1 hypothetical protein -
CJ258_RS27140 98991..99194 + 204 WP_004152106.1 hemolysin expression modulator Hha -
CJ258_RS27145 99243..99500 + 258 WP_004152107.1 hypothetical protein -
CJ258_RS27150 99576..99830 + 255 WP_004152108.1 hypothetical protein -
CJ258_RS27155 100006..100272 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CJ258_RS27160 100260..100742 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CJ258_RS27165 100950..102296 + 1347 WP_077253535.1 ISNCY family transposase -
CJ258_RS27170 102345..102740 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
CJ258_RS27175 102888..104142 - 1255 Protein_101 IS3 family transposase -
CJ258_RS27180 104230..105192 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / blaOXA-1 / tet(A) / qnrB1 - 1..258210 258210


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T80241 WP_003026803.1 NZ_CP022926:100260-100742 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T80241 NZ_CP022926:100260-100742 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT80241 WP_003026799.1 NZ_CP022926:100006-100272 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT80241 NZ_CP022926:100006-100272 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References