Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4002635..4003254 Replicon chromosome
Accession NZ_CP022924
Organism Klebsiella pneumoniae strain ST307PT01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CJ258_RS19950 Protein ID WP_002892050.1
Coordinates 4003036..4003254 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CJ258_RS19945 Protein ID WP_002892066.1
Coordinates 4002635..4003009 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ258_RS19935 3997787..3998980 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CJ258_RS19940 3999003..4002149 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CJ258_RS19945 4002635..4003009 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CJ258_RS19950 4003036..4003254 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CJ258_RS19955 4003413..4003979 + 567 WP_002892030.1 maltose O-acetyltransferase -
CJ258_RS29005 4003951..4004091 - 141 WP_004147370.1 hypothetical protein -
CJ258_RS19960 4004112..4004582 + 471 WP_002892026.1 YlaC family protein -
CJ258_RS19965 4004557..4006008 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
CJ258_RS19970 4006109..4006807 + 699 WP_032435564.1 GNAT family N-acetyltransferase -
CJ258_RS19975 4006804..4006944 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CJ258_RS19980 4006944..4007207 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T80235 WP_002892050.1 NZ_CP022924:4003036-4003254 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T80235 NZ_CP022924:4003036-4003254 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT80235 WP_002892066.1 NZ_CP022924:4002635-4003009 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT80235 NZ_CP022924:4002635-4003009 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References