Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 77932..78575 Replicon plasmid pJYC02A
Accession NZ_CP022923
Organism Klebsiella pneumoniae strain ST307PT02

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CJ259_RS26920 Protein ID WP_001044770.1
Coordinates 77932..78348 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CJ259_RS26925 Protein ID WP_001261282.1
Coordinates 78345..78575 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ259_RS26885 73474..73710 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
CJ259_RS26890 73707..74069 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
CJ259_RS26895 74087..75781 - 1695 WP_000105636.1 mercury(II) reductase -
CJ259_RS26900 75833..76255 - 423 WP_001340589.1 organomercurial transporter MerC -
CJ259_RS26905 76291..76401 - 111 Protein_88 mercuric transport protein periplasmic component -
CJ259_RS26910 76417..77114 - 698 Protein_89 IS1 family transposase -
CJ259_RS26915 77148..77858 - 711 WP_004152334.1 ATP-binding protein -
CJ259_RS26920 77932..78348 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CJ259_RS26925 78345..78575 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CJ259_RS26930 78532..78993 + 462 WP_072093212.1 hypothetical protein -
CJ259_RS26940 79480..79788 + 309 WP_004152336.1 hypothetical protein -
CJ259_RS26945 79816..80145 + 330 WP_004152337.1 hypothetical protein -
CJ259_RS26950 80171..80569 + 399 WP_004171440.1 hypothetical protein -
CJ259_RS26955 80576..80908 + 333 WP_004152339.1 hypothetical protein -
CJ259_RS26960 80908..81690 + 783 WP_004152340.1 site-specific integrase -
CJ259_RS26965 82582..82812 - 231 WP_011977773.1 hypothetical protein -
CJ259_RS26970 82904..83377 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / tet(A) / qnrB1 / blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..246577 246577
- flank IS/Tn - - 83497..84765 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T80221 WP_001044770.1 NZ_CP022923:c78348-77932 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T80221 NZ_CP022923:c78348-77932 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT80221 WP_001261282.1 NZ_CP022923:c78575-78345 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT80221 NZ_CP022923:c78575-78345 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References