Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 18247..18983 Replicon plasmid pJYC02A
Accession NZ_CP022923
Organism Klebsiella pneumoniae strain ST307PT02

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CJ259_RS26550 Protein ID WP_003026803.1
Coordinates 18501..18983 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CJ259_RS26545 Protein ID WP_003026799.1
Coordinates 18247..18513 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ259_RS26500 14309..14671 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
CJ259_RS26505 14721..15071 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CJ259_RS26510 15429..15698 + 270 WP_004152102.1 hypothetical protein -
CJ259_RS26515 15686..16261 + 576 WP_004152103.1 hypothetical protein -
CJ259_RS26520 16292..16786 + 495 WP_004152104.1 DNA-binding protein -
CJ259_RS26525 16830..17198 + 369 WP_004152105.1 hypothetical protein -
CJ259_RS26530 17232..17435 + 204 WP_004152106.1 hemolysin expression modulator Hha -
CJ259_RS26535 17484..17741 + 258 WP_004152107.1 hypothetical protein -
CJ259_RS26540 17817..18071 + 255 WP_004152108.1 hypothetical protein -
CJ259_RS26545 18247..18513 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CJ259_RS26550 18501..18983 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CJ259_RS26555 19191..20537 + 1347 WP_077253535.1 ISNCY family transposase -
CJ259_RS26560 20586..20981 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
CJ259_RS26565 21129..22383 - 1255 Protein_29 IS3 family transposase -
CJ259_RS26570 22471..23433 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / tet(A) / qnrB1 / blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..246577 246577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T80220 WP_003026803.1 NZ_CP022923:18501-18983 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T80220 NZ_CP022923:18501-18983 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT80220 WP_003026799.1 NZ_CP022923:18247-18513 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT80220 NZ_CP022923:18247-18513 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References