Detailed information of TA system TA080216

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4668850..4669553
• Replicon: chromosome
• Accession: NZ_CP022922
• Organism: Klebsiella pneumoniae strain ST307PT02

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A939NMR5
• Locus tag: CJ259_RS23185
• Protein ID: WP_071994632.1
• Coordinates: 4668850..4669191 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A939NIK9
• Locus tag: CJ259_RS23190
• Protein ID: WP_032434296.1
• Coordinates: 4669212..4669553 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJ259_RS23175	4665165..4666034	+	870	WP_023317468.1	HNH endonuclease	-
CJ259_RS23180	4666625..4668658	+	2034	WP_050598589.1	hypothetical protein	-
CJ259_RS23185	4668850..4669191	-	342	WP_071994632.1	TA system toxin CbtA family protein	Toxin
CJ259_RS23190	4669212..4669553	-	342	WP_032434296.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CJ259_RS23195	4669564..4670106	-	543	WP_032434298.1	DNA repair protein RadC	-
CJ259_RS23200	4670119..4670559	-	441	WP_032434300.1	antirestriction protein	-
CJ259_RS23205	4670590..4671411	-	822	WP_032434301.1	DUF945 domain-containing protein	-
CJ259_RS23210	4671531..4672004	-	474	WP_032434303.1	hypothetical protein	-
CJ259_RS23215	4672076..4672528	-	453	WP_032410767.1	hypothetical protein	-
CJ259_RS23220	4672564..4673280	-	717	WP_032434305.1	WYL domain-containing protein	-
CJ259_RS23225	4673524..4674398	-	875	Protein_4455	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4657147..4702820	45673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12789.77 Da
• Isoelectric Point: 9.6552

>T80216 WP_071994632.1 NZ_CP022922:c4669191-4668850 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRKGF
NWQEHSPYLRAVDILRARQATGLLRRSRKNAVR

Nucleotide

• Length: 342 bp

>T80216 NZ_CP022922:c4669191-4668850 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12893.40 Da
• Isoelectric Point: 5.9612

>AT80216 WP_032434296.1 NZ_CP022922:c4669553-4669212 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGEFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHFFTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT80216 NZ_CP022922:c4669553-4669212 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA