Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 167553..168289 Replicon plasmid pJYC03A
Accession NZ_CP022920
Organism Klebsiella pneumoniae strain ST307PT03

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CJ256_RS27500 Protein ID WP_003026803.1
Coordinates 167553..168035 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CJ256_RS27505 Protein ID WP_003026799.1
Coordinates 168023..168289 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ256_RS27480 163103..164065 + 963 WP_004152113.1 M48 family metalloprotease -
CJ256_RS27485 164153..165407 + 1255 Protein_186 IS3 family transposase -
CJ256_RS27490 165555..165950 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
CJ256_RS27495 165999..167345 - 1347 WP_077253535.1 ISNCY family transposase -
CJ256_RS27500 167553..168035 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CJ256_RS27505 168023..168289 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CJ256_RS27510 168465..168719 - 255 WP_004152108.1 hypothetical protein -
CJ256_RS27515 168795..169052 - 258 WP_004152107.1 hypothetical protein -
CJ256_RS27520 169101..169304 - 204 WP_004152106.1 hemolysin expression modulator Hha -
CJ256_RS27525 169338..169706 - 369 WP_004152105.1 hypothetical protein -
CJ256_RS27530 169750..170244 - 495 WP_004152104.1 DNA-binding protein -
CJ256_RS27535 170275..170850 - 576 WP_004152103.1 hypothetical protein -
CJ256_RS27540 170838..171107 - 270 WP_004152102.1 hypothetical protein -
CJ256_RS27545 171465..171815 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CJ256_RS27550 171865..172227 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / qnrB1 / tet(A) / dfrA14 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / blaOXA-9 - 1..246577 246577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T80200 WP_003026803.1 NZ_CP022920:c168035-167553 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T80200 NZ_CP022920:c168035-167553 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT80200 WP_003026799.1 NZ_CP022920:c168289-168023 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT80200 NZ_CP022920:c168289-168023 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References