Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 107961..108604 Replicon plasmid pJYC03A
Accession NZ_CP022920
Organism Klebsiella pneumoniae strain ST307PT03

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CJ256_RS27130 Protein ID WP_001044770.1
Coordinates 108188..108604 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CJ256_RS27125 Protein ID WP_001261282.1
Coordinates 107961..108191 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ256_RS27080 103159..103632 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
CJ256_RS27085 103724..103954 + 231 WP_011977773.1 hypothetical protein -
CJ256_RS27090 104846..105628 - 783 WP_004152340.1 site-specific integrase -
CJ256_RS27095 105628..105960 - 333 WP_004152339.1 hypothetical protein -
CJ256_RS27100 105967..106365 - 399 WP_004171440.1 hypothetical protein -
CJ256_RS27105 106391..106720 - 330 WP_004152337.1 hypothetical protein -
CJ256_RS27110 106748..107056 - 309 WP_004152336.1 hypothetical protein -
CJ256_RS27120 107543..108004 - 462 WP_072093212.1 hypothetical protein -
CJ256_RS27125 107961..108191 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CJ256_RS27130 108188..108604 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CJ256_RS27135 108678..109388 + 711 WP_004152334.1 ATP-binding protein -
CJ256_RS27140 109422..110119 + 698 Protein_126 IS1 family transposase -
CJ256_RS27145 110135..110245 + 111 Protein_127 mercuric transport protein periplasmic component -
CJ256_RS27150 110281..110703 + 423 WP_001340589.1 organomercurial transporter MerC -
CJ256_RS27155 110755..112449 + 1695 WP_000105636.1 mercury(II) reductase -
CJ256_RS27160 112467..112829 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
CJ256_RS27165 112826..113062 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / qnrB1 / tet(A) / dfrA14 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / blaOXA-9 - 1..246577 246577
- flank IS/Tn - - 101771..103039 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T80199 WP_001044770.1 NZ_CP022920:108188-108604 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T80199 NZ_CP022920:108188-108604 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT80199 WP_001261282.1 NZ_CP022920:107961-108191 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT80199 NZ_CP022920:107961-108191 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References