Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59432..60075 Replicon plasmid pJYC04A
Accession NZ_CP022917
Organism Klebsiella pneumoniae strain ST307PT04

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CJ257_RS26820 Protein ID WP_001044770.1
Coordinates 59432..59848 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CJ257_RS26825 Protein ID WP_001261282.1
Coordinates 59845..60075 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ257_RS26785 54974..55210 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
CJ257_RS26790 55207..55569 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
CJ257_RS26795 55587..57281 - 1695 WP_000105636.1 mercury(II) reductase -
CJ257_RS26800 57333..57755 - 423 WP_001340589.1 organomercurial transporter MerC -
CJ257_RS26805 57791..57901 - 111 Protein_63 mercuric transport protein periplasmic component -
CJ257_RS26810 57917..58614 - 698 Protein_64 IS1 family transposase -
CJ257_RS26815 58648..59358 - 711 WP_004152334.1 ATP-binding protein -
CJ257_RS26820 59432..59848 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CJ257_RS26825 59845..60075 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CJ257_RS26830 60032..60493 + 462 WP_072093212.1 hypothetical protein -
CJ257_RS26840 60980..61288 + 309 WP_004152336.1 hypothetical protein -
CJ257_RS26845 61316..61645 + 330 WP_004152337.1 hypothetical protein -
CJ257_RS26850 61671..62069 + 399 WP_004171440.1 hypothetical protein -
CJ257_RS26855 62076..62408 + 333 WP_004152339.1 hypothetical protein -
CJ257_RS26860 62408..63190 + 783 WP_119948544.1 site-specific integrase -
CJ257_RS26865 64082..64312 - 231 WP_011977773.1 hypothetical protein -
CJ257_RS26870 64404..64877 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / tet(A) / qnrB1 / blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..246577 246577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T80179 WP_001044770.1 NZ_CP022917:c59848-59432 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T80179 NZ_CP022917:c59848-59432 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT80179 WP_001261282.1 NZ_CP022917:c60075-59845 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT80179 NZ_CP022917:c60075-59845 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References