Detailed information of TA system TA080174

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4673649..4674352
• Replicon: chromosome
• Accession: NZ_CP022916
• Organism: Klebsiella pneumoniae strain ST307PT04

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A939NMR5
• Locus tag: CJ257_RS23205
• Protein ID: WP_071994632.1
• Coordinates: 4673649..4673990 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A939NIK9
• Locus tag: CJ257_RS23210
• Protein ID: WP_032434296.1
• Coordinates: 4674011..4674352 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJ257_RS23195	4669964..4670833	+	870	WP_023317468.1	HNH endonuclease	-
CJ257_RS23200	4671424..4673457	+	2034	WP_050598589.1	hypothetical protein	-
CJ257_RS23205	4673649..4673990	-	342	WP_071994632.1	TA system toxin CbtA family protein	Toxin
CJ257_RS23210	4674011..4674352	-	342	WP_032434296.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CJ257_RS23215	4674363..4674905	-	543	WP_032434298.1	DNA repair protein RadC	-
CJ257_RS23220	4674918..4675358	-	441	WP_032434300.1	antirestriction protein	-
CJ257_RS23225	4675389..4676210	-	822	WP_032434301.1	DUF945 domain-containing protein	-
CJ257_RS23230	4676330..4676803	-	474	WP_032434303.1	hypothetical protein	-
CJ257_RS23235	4676875..4677327	-	453	WP_032410767.1	hypothetical protein	-
CJ257_RS23240	4677363..4678079	-	717	WP_032434305.1	WYL domain-containing protein	-
CJ257_RS23245	4678323..4679197	-	875	Protein_4461	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4661946..4707620	45674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12789.77 Da
• Isoelectric Point: 9.6552

>T80174 WP_071994632.1 NZ_CP022916:c4673990-4673649 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRKGF
NWQEHSPYLRAVDILRARQATGLLRRSRKNAVR

Nucleotide

• Length: 342 bp

>T80174 NZ_CP022916:c4673990-4673649 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12893.40 Da
• Isoelectric Point: 5.9612

>AT80174 WP_032434296.1 NZ_CP022916:c4674352-4674011 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGEFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHFFTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT80174 NZ_CP022916:c4674352-4674011 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA