Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 68340..68865 Replicon plasmid unnamed2
Accession NZ_CP022914
Organism Escherichia coli O6:H16 strain 2011EL-1370-2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CJZ79_RS26020 Protein ID WP_001159871.1
Coordinates 68340..68645 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CJZ79_RS26025 Protein ID WP_000813634.1
Coordinates 68647..68865 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJZ79_RS26000 64585..65288 - 704 Protein_70 helix-turn-helix domain-containing protein -
CJZ79_RS26005 65536..66215 + 680 Protein_71 EAL domain-containing protein -
CJZ79_RS26010 66655..67296 - 642 WP_001705742.1 recombinase family protein -
CJZ79_RS26015 67626..68339 - 714 WP_000016957.1 site-specific integrase -
CJZ79_RS26020 68340..68645 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CJZ79_RS26025 68647..68865 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CJZ79_RS26040 69310..69567 + 258 WP_000343092.1 hypothetical protein -
CJZ79_RS26045 69567..70097 + 531 WP_011387704.1 hypothetical protein -
CJZ79_RS26050 70341..71183 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
CJZ79_RS26055 71186..72274 + 1089 WP_064734538.1 putative hexosyltransferase CapU -
CJZ79_RS26060 72279..73229 + 951 WP_032328199.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - etpB / etpA / espC / eltB / eltA / cs3 1..117021 117021
- flank IS/Tn - - 66655..67278 623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T80155 WP_001159871.1 NZ_CP022914:c68645-68340 [Escherichia coli O6:H16]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T80155 NZ_CP022914:c68645-68340 [Escherichia coli O6:H16]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT80155 WP_000813634.1 NZ_CP022914:c68865-68647 [Escherichia coli O6:H16]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT80155 NZ_CP022914:c68865-68647 [Escherichia coli O6:H16]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References